engleski

Mutual dendritic cell/NK interaction leads to their well balanced co-existence at the maternal-fetal interface

Dendritic cell/NK (DC/NK) inter-reactions of the early human pregnancy deciduas were analyzed in vitro. The characterization of CD1a+ and CD83+ cells was performed in the suspension of freshly isolated decidual mononuclear cells (DMC) in terms of phenotype and intracellular cytokine detection. Intermediate HLA-DR expression, low co-stimulatory (CD80, CD86) and significant endocytic molecule expression with the presence of CCR5 chemokine receptor, CD14 and CD56 indicated immature phenotype of CD1a+ cells. CD83+ subpopulation showed more mature phenotype and lower percentage of IL-15 and IFN-gamma producing cells then CD1a+ cells. The magnetic separation of CD56+ and CD83+ cells from the non-adherent and CD1a+ from the adherent DMC fraction was established. Purified CD56+ cells were cultured in the medium only or co-cultured with enriched CD1a+ or CD83+ cells. The proliferation, NK cell receptors, intracellular cytokines and cytolytic mediators expression in CD56+ bright cells have been analyzed. Immature CD1a+ cells increased perforin, FasL and TRAIL expression in NK cells, but decreased NKp46 receptor expression and pro-inflammatory cytokine production (18 hours). CD83+ cells did not show such effects. CD1a+ cells caused higher proliferation rate of decidual CD56+ bright cells, then CD83+ cells. Both dendritic subpopulations can be killed by decidual CD56+ cells, measured by 2 hrs PKH-26 (red) cytotoxicity assay. It seems that mutual DC/NK interaction leads to their well balanced co-existence at the maternal-fetal interface. Acknowledgement: The experiments were financed by the grants of EMBIC European FP6 project No. 512040, LSHM-CT-2004-512040 and Croatian Ministry of Science, Education and Sports No. 0376 and No. 0377.

dendritic cells; human pregnancy; decidua

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