Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 309210

Development and characterization of P-glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line


Žaja, Roko; Caminada, Daniel; Lončar, Jovica; Fent, Karl; Smital, Tvrtko
Development and characterization of P-glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line // Toxicology and Applied Pharmacology, 227 (2008), 2; 207-218 doi:10.1016/j.taap.2007.11.001 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 309210 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Development and characterization of P-glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line

Autori
Žaja, Roko ; Caminada, Daniel ; Lončar, Jovica ; Fent, Karl ; Smital, Tvrtko

Izvornik
Toxicology and Applied Pharmacology (0041-008X) 227 (2008), 2; 207-218

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Pgp1 ; fish cells ; MXR ; MDR ; induction

Sažetak
The development of the multidrug resistance (MDR) phenotype in mammals is often mediated by the overexpression of the P-glycoprotein1 (Pgp, ABCB1) or multidrug resistance associated protein (MRP)-like ABC transport proteins. A similar phenomenon has also been observed and considered as an important part of the multixenobiotic resistance (MXR) defence system in aquatic organisms. We have recently demonstrated the presence of ABC transporters in the widely used in vitro fish model, the PLHC-1 hepatoma cell line. In the present study we were able to select a highly resistant PLHC-1 sub-clone (PLHC-1/dox) by culturing the wild type cells in the presence of 1 µ ; ; ; M doxorubicin. Using quantitative PCR a 42-fold higher expression of ABCB1 gene was determined in the PLHC-1/dox cells compared to non-selected wild type cells (PLHC-1/wt). The efflux rates of model fluorescent Pgp1 substrates rhodamine 123 and calcein-AM were 3- to 4-fold higher in the PLHC-1/dox in comparison to the PLHC-1/wt cells. PLHC-1/dox were 45-fold more resistant to doxorubicin cytotoxicity than PLHC-1/wt. Similarly to mammalian cell lines, typical cross-resistance to cytotoxicity of other chemotherapeutics such as daunorubicin, vincristine, vinblastine, etoposide and colchicine, occurred. Furthermore, cyclosporine A, verapamil and PSC833, specific inhibitors of Pgp1 transport activity, completely reversed resistance of PLHC-1/dox cells to all tested drugs, resulting in EC50 values similar to the EC50 values found for PLHC-1/wt. In contrast, MK571, a specific inhibitor of MRP type of efflux transporters, sensitized PLHC-1/dox cells, neither to doxorubicin, nor to any other of the chemotherapeutics used in the study. These data demonstrate for the first time that a specific, Pgp1 mediated doxorubicin resistance mechanism is present in the PLHC-1 fish hepatoma cell line. In addition, the fact that low micromolar concentrations of specific inhibitors may completely reverse a highly expressed doxorubicin resistance points to the fragility of Pgp1 mediated MXR defence mechanism in fish.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
098-0982934-2745 - Ekotoksikološko značenje ABC transportnih proteina u vodenih organizama (Smital, Tvrtko, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Jovica Lončar (autor)

Avatar Url Roko Žaja (autor)

Avatar Url Tvrtko Smital (autor)

Citiraj ovu publikaciju

Žaja, Roko; Caminada, Daniel; Lončar, Jovica; Fent, Karl; Smital, Tvrtko
Development and characterization of P-glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line // Toxicology and Applied Pharmacology, 227 (2008), 2; 207-218 doi:10.1016/j.taap.2007.11.001 (međunarodna recenzija, članak, znanstveni)
Žaja, R., Caminada, D., Lončar, J., Fent, K. & Smital, T. (2008) Development and characterization of P-glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line. Toxicology and Applied Pharmacology, 227 (2), 207-218 doi:10.1016/j.taap.2007.11.001.
@article{article, year = {2008}, pages = {207-218}, DOI = {10.1016/j.taap.2007.11.001}, keywords = {Pgp1, fish cells, MXR, MDR, induction}, journal = {Toxicology and Applied Pharmacology}, doi = {10.1016/j.taap.2007.11.001}, volume = {227}, number = {2}, issn = {0041-008X}, title = {Development and characterization of P-glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line}, keyword = {Pgp1, fish cells, MXR, MDR, induction} }
@article{article, year = {2008}, pages = {207-218}, DOI = {10.1016/j.taap.2007.11.001}, keywords = {Pgp1, fish cells, MXR, MDR, induction}, journal = {Toxicology and Applied Pharmacology}, doi = {10.1016/j.taap.2007.11.001}, volume = {227}, number = {2}, issn = {0041-008X}, title = {Development and characterization of P-glycoprotein 1 (Pgp1 ; ABCB1) mediated doxorubicin-resistant PLHC-1 hepatoma fish cell line}, keyword = {Pgp1, fish cells, MXR, MDR, induction} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font