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Expression of 5HT-1A and 5HT-1B receptor genes in brains of Wistar-Zagreb 5HT rats

By selective breeding, two sublines of rats with high or low activity of platelet serotonin transporter (5HTt) have been developed (Wistar-Zagreb 5HT rats). Previous studies demonstrated significant differences between sublines in the expression of platelet 5HTt at the level of both, mRNA and protein. Analysis of brain 5HTt gene expression did not show analogous differences, although pharmacological studies showed marked alterations in brain 5HTt function, indicating differences in central 5HT homeostasis. In this study, we searched for possible changes in the expression of two central 5HT receptor subtypes: 5HT-1A and 5HT-1B, both participating in the regulation of brain 5HT transmission. Semi-quantitative RT-PCR, with three different housekeeping genes as internal standards, showed no differences in 5HT-receptor messages between sublines. Results suggest that constitutional alteration of serotonergic transmission, induced by inherited dysregulation of the 5HT transporter, did not cause measurable changes in the expression of central 5HT-1A (hippocampus) and 5HT-1B (striatum) receptors in the mentioned rat sublines.

serotonin receptor ; gene expression ; rat brain

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