engleski

Pharmagological active volatile compounds from some aromatic plants

Reactive oxygen species, which are generated during metabolic process of aerobic cell life, cause the damage of various macromolecules like proteins, nucleic acids and lipids. Cells have the natural mechanism for antioxidant defense as the prevention of reactive oxygen species. However, very often this defense is not enough, and the consequence is characterized with numerous chronic diseases like atherosclerosis, rheumatic diseases, tumors and different neurodegenerative diseases like Alzheimer's and Parkin's diseases. At present it is well known that the control of oxidative processes in living organisms is possible by diverse antioxidants ingested into organisms with foods. For this reason there is a growing interest in the investigations of biologic active natural compounds from medicinal plants. The goal of these investigations are the isolation, fractionation, identification and examination of biologic activities of their volatile components. During this communication it will be presented the investigation of some aromatic plants in purpose to reveal their biologic activity (antioxidant potential, inhibitor properties on LDL proteins, acetylcholinesterase and antitumor activity). In process of isolation and identification of antioxidant compounds, the conventional extraction methods have been used. Separation of active components is performed using chromatographic techniques. Identification of chemical composition and amount of volatile compounds has been performed using chromatography and spectrometry (GC-MS, HPLC, UV/VIS). Antioxidant activity is examined using following standard methods: DPPH radical scavenging, FRAP the ferric reducing ability of plasma, TBARS method with thiobarbituic acid and RANCIMAT automatic method for identification of oxidative stability of fats and oils. Research on acetylcholinesterase activity, in absence and presence of inhibitory compounds, is performed with standard UV/VIS spectrophotometric methods. Antitumor activity is demonstrated in vitro and in vivo using one L929 mouse fibroblasts and two tumor cell lines: squamous cell carcinoma - SCC VII and fibrosarcoma - FsaR. It was showed that aromatic plants possess biologic active volatile compounds with antioxidant, inhibitor properties on LDL proteins, acetylcholinesterase and antitumor activity. They are presents as free volatiles in essential oil or as hidden potential in form of volatile aglycones which are glycosidically bound on sugar unities. Their biologic activity is demonstrated after catalytic transformation or hydrolysis.

Reactive oxygen species; neurodegenerative diseases

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