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Pregled bibliografske jedinice broj: 30691

Analysis of clonality in T-lymphoproliferative diseases by multiplex PCR


Zadro, Renata; Sučić, Mirna; Aurer, Igor; Metelko-Kovačević, Jasminka; Labar, Boris; Stavljenić-Rukavina, Ana
Analysis of clonality in T-lymphoproliferative diseases by multiplex PCR // Clinical chemistry and laboratory medicine, 36 (1998), 8; 637-639 doi:10.1515/CCLM.1998.112 (međunarodna recenzija, članak, znanstveni)


Naslov
Analysis of clonality in T-lymphoproliferative diseases by multiplex PCR

Autori
Zadro, Renata ; Sučić, Mirna ; Aurer, Igor ; Metelko-Kovačević, Jasminka ; Labar, Boris ; Stavljenić-Rukavina, Ana

Izvornik
Clinical chemistry and laboratory medicine (1434-6621) 36 (1998), 8; 637-639

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
T-cell receptor gamma gene rearrangement ; monoclonality ; lineage ; polymerase chain reaction

Sažetak
Distinction between benign and malignant T-cell lymphoproliferative disease can be difficult using morphological criteria. Using multiplex PCR system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T- lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal PCR products. By splitting the multiplex primer mix the patient specific TCRgamma rearrangement was determined: two of ten patients showed the exclusive presence of a single TCRgamma gene rearrangement. Three patients exhibited two rearranged TCRgamma genes, while in five patients positive reactions were obtained with three VJ primer combinations. Molecular analysis of rearranged T-cell receptor genes by multiplex PCR represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
129-0000000-3385 - Citološki pokazatelji proliferacije stanica (Mirna Sučić, )
214006

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Medicinski fakultet, Zagreb,
Klinička bolnica "Sveti Duh",
Klinički bolnički centar Zagreb,
Sveučilište Libertas

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • Excerpta Medica
  • Index Medicus
  • Medline
  • SCIex


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