engleski

Wistar-Zagreb 5HT rat : further progress in development of the model

Serotonergic system possess a peculiarity as compared to the other neurotransmitter/neuromodulator systems that consists in the peripheral expression of three relevant synaptic proteins on accessible blood elements - transporter (5HTt), postsynaptic receptor (5HT-2A) and metabolising enzyme (MAO-B). By selective breeding of animals for the extremes of transporter velocities, two sublines of rats markedly differing in platelet 5HT transporter kinetics were developed. The model was termed Wistar:Zagreb-5HT rats (WZ-5HT rats). The obtained constitutional dysregulation of platelet 5HTt was paralleled with analogous dysregulations of its central counterpart (i.e. presynaptic membrane transporter mediating 5HT reuptake), as strongly indicated by neurochemical, behavioural and molecular indicators. Taking into account a pivotal role which 5HTt holds in the regulation of serotonergic transmission, the mentioned dysregulation provoked behavioural alterations, as expected. On the other hand, alterations in neurochemical and molecular synaptic parameters that have also been expected, were not so easy demonstrable under physiological conditions. Results of both sets of experiments will be presented.

serotonergic system; 5HT transporter; platelet; brain

nije evidentirano