engleski

Impact of 864 MHz or 935 MHz radiofrequency microwave radiation on the basic growth parameters of V79 cell line

Aim of study was to evaluate and compare influence of two different radiofrequency/microwave (RF/MW) fields of 864 and 935 MHz frequency on the growth, colony-forming ability and viability of continuous cell line V79. Cell samples in concentration of 1x104 each, were exposed to continuous wave frequencies of 864 and 935 MHz for 1, 2 and 3 hours. Exposed samples were matched with sham-exposed controls. Specific absorption rate (SAR) was calculated to be 0.08 W/kg for 864 MHz or 0.12 W/kg for 935 MHz field. Cell growth and viability was determined by cell counts every day during five days after exposure. Colony-forming ability was assessed by colony number seven days after exposure. The growth of the 864 MHz irradiated cells showed significant decrease after two- and three-hour exposure 72 hours after irradiation (p<0.05). Cells exposed to 935 MHz microwaves for three hours showed significant decrease 72 hours after exposure (p<0.05). Colony-forming ability and cell viability exposed to 864 MHz or 935 MHz microwaves did not significantly differ from the controls. The applied RF/MW fields showed similar effect on growth, colony-forming ability and viability of V79 cells. Cell growth findings reveal time-dependent influence of both, 864 and 935 MHz frequency field.

V79 cells; exposure; 864 MHz; 935 MHz; growth; colony-forming ability; viability

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