engleski

HuIFN-Alpha N3 and PoIFN-Beta affect the replication of Avian Influenza (H5N2) virus in the embryonated chicken egg

Avian influenza (AI) H5N1 virus, the extremely contagious strain, can be deadly to domestic poultry. Without &raquo ; ; jumping&laquo ; ; the species barrier it can seldom cause infections in humans. Such an infection coincided with devastating epidemics in poultry farms in Asian countries, with the reported mortality approaching 100%. Closely related to this highly pathogenic avian influenza (HPAI) is strain H5N2, being antigenically similar to H5N1 although distinguishable from an influenza H5N1 virus isolated from a human in Hong Kong. Nevertheless phylogenetic analysis of the haemagglutinin (HA) genes showed that the highly pathogenic H5N2 clustered with the human H5N1 virus isolated in Hong Kong, even though up to now, there are no reports of the transmission of this virus from bird to human. The presented experiments were aimed to find out if low doses of HuIFN-Alpha N3 and PoIFN-Beta affect the replication of AI (H5N2) virus in the embryonated chicken eggs and through this the survival of the infected embryos. The obtained results were evaluated on the basis of the EID50 value: 10-4.249/0.1 ml, and where the following: virus (10– 4) + IFNs given simultaneously shows the effectiveness of low doses (150 I.U.) of IFN, resulting in 100% embryo survival and 100% HA inhibition. Virus (10– 4)+ IFNs given after 2h: only the high doses (15.000) are in some way effective (33.3% survival). IFNs + virus (10– 4) after 2h are probably more important, because this pre-treatment with low doses and infection after 2 hours resulted in a decrease of HA as well as 66.7% survival. This is probably connected with the neuraminidase (NA) inactivation. Further experiments will show how to optimize the effectiveness of HuIFN-Alpha N3 and PoIFN-Beta toward the resistance of AI (H5N2) virus with different effectors like different l-amino acids.

Avian Influenza (H5N1); Avian Influenza (H5N2); HuIFN-Alpha N3; PoIFN-Beta; Chicken embryo; Haemagglutination; Embryo survival

nije evidentirano