engleski

Genotoxicity and mutagenicity of tryptophol from Candida albicans

Tryptophol (3-indoleethanol) is a low-molecular-weight aromatic alcohol produced by various fungi, including opportunistic Candida species and Trypanosoma sp. parasite. It can be found in beverages such as beer, wine as well as in olive oil. The aim of this study was to explore the mutagenicity and genotoxicity of tryptophol in vitro. Genotoxic effects were studied on epithelial cell lines (A549, Hep G-2, CHO), monocyte cell line (THP-1) and human peripheral blood lymphocytes (LPB). Furthermore, mutagenic effect was tested on Salmonella enterica serovar Typhimurium TA98 and TA100 without S9 fraction using Ames test. Sub-cytotoxic concentrations of tryptophol (0.25 to 2 mM) caused significant dose-depended changes in tail length, percent of DNA and tail moment of LPB. The concentration of 2 mM demonstrated cell-line depended genotoxic effect. A549 cell line was the most sensitive while CHO cell line has been unchanged at the same concentration. No mutagenic effect was found using Ames assay at maximum concentration of 1.6 mg/plate. Our study showed that subcytotoxic concentrations of tryptophol cause genotoxic but not mutagenic effects.

candida; tryptophol; come assay; Ames test

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