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Genotoxicity and mutagenicity of tryptophol from Candida albicans


Kosalec, Ivan; Kopjar, Nevenka; Ramić, Snježana; Bačun-Družina, Višnja
Genotoxicity and mutagenicity of tryptophol from Candida albicans // Power of Microbes in Industry and Environment: Programme and Abstracts / Kosalec, Ivan ; Pigac, Jasenka ; Vujaklija, Dušica (ur.).
Zagreb: Hrvatsko mikrobiološko društvo, 2007. (poster, nije recenziran, sažetak, znanstveni)


Naslov
Genotoxicity and mutagenicity of tryptophol from Candida albicans

Autori
Kosalec, Ivan ; Kopjar, Nevenka ; Ramić, Snježana ; Bačun-Družina, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of Microbes in Industry and Environment: Programme and Abstracts / Kosalec, Ivan ; Pigac, Jasenka ; Vujaklija, Dušica - Zagreb : Hrvatsko mikrobiološko društvo, 2007

ISBN
978-953-96567-5-9

Skup
Power of Microbes in Industry and Environment

Mjesto i datum
Zadar, Hrvatska, 19-22.9.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Candida; tryptophol; come assay; Ames test

Sažetak
Tryptophol (3-indoleethanol) is a low-molecular-weight aromatic alcohol produced by various fungi, including opportunistic Candida species and Trypanosoma sp. parasite. It can be found in beverages such as beer, wine as well as in olive oil. The aim of this study was to explore the mutagenicity and genotoxicity of tryptophol in vitro. Genotoxic effects were studied on epithelial cell lines (A549, Hep G-2, CHO), monocyte cell line (THP-1) and human peripheral blood lymphocytes (LPB). Furthermore, mutagenic effect was tested on Salmonella enterica serovar Typhimurium TA98 and TA100 without S9 fraction using Ames test. Sub-cytotoxic concentrations of tryptophol (0.25 to 2 mM) caused significant dose-depended changes in tail length, percent of DNA and tail moment of LPB. The concentration of 2 mM demonstrated cell-line depended genotoxic effect. A549 cell line was the most sensitive while CHO cell line has been unchanged at the same concentration. No mutagenic effect was found using Ames assay at maximum concentration of 1.6 mg/plate. Our study showed that subcytotoxic concentrations of tryptophol cause genotoxic but not mutagenic effects.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
006-0061117-1242 - Mikromicete, interakcije toksičnih metabolita-zdravlje i prevencija (Maja Šegvić Klarić, )
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb