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Vegetativno razmnožavanje vrste Epimedium alpinum L. u uvjetima in vitro (CROSBI ID 529758)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija

Mihaljević, Snježana ; Vršek, Ines Vegetativno razmnožavanje vrste Epimedium alpinum L. u uvjetima in vitro / Britvec, Mihaela ; Škvorc, Željko (ur.). Zagreb: Hrvatsko Botaničko Društvo, 2007. str. 116-117-x

Podaci o odgovornosti

Mihaljević, Snježana ; Vršek, Ines

hrvatski

Vegetativno razmnožavanje vrste Epimedium alpinum L. u uvjetima in vitro

The genus Epimedium (bishop’ s hat, barrenwort) in the family Berberidaceae comprises about 60 species of rhizomatous perennials. A number of species, hybrids, and cultivars are grown as ornamentals in woodland gardens and naturalized areas. In Croatia, Epimedium alpinum L. has the status of a vulnerable species endangered by uncontrolled collecting. The plant can be propagated by division of its long rhizomes or by seeds. The difficulties associated with gathering the seeds and low seed viability make bishop’ s hat a suitable species for propagation by tissue culture. In an attempt to develop a system for micropropagation of E. alpinum, the effects of various concentrations of thidiazuron (TDZ) and N-(2-chloro-4-pyridyl)-N'-phenylurea (CPPU), on the induction of organogenic callus was evaluated. Organogenesis from immature seeds occurred most efficiently when they were transiently exposed (48 hours) to 20 &micro ; M CPPU or 80 &micro ; M TDZ followed by culture on hormone-free woody-plant medium (WPM). Organogenic callus consisting of white, compact clumps of tissue proliferated slowly on hormone-free WPM. To promote adventitious shoot induction, the effects of different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzyladenine (BA) were investigated. WPM containing 1.1 &micro ; M 2, 4-D and 4.4 &micro ; M BA was optimal for callus proliferation while shoot induction and elongation was enhanced on media with 1.1 &micro ; M 2, 4-D and 2.2 or 22 &micro ; M BA. Shoots were successfully rooted on hormone-free WPM and well-developed plantlets were transferred to soil.

kultura tkiva; organogeneza; regeneracija

nije evidentirano

engleski

In vitro propagation of Epimedium alpinum L.

nije evidentirano

organogenesis; regeneration; tissue culture

nije evidentirano

Podaci o prilogu

116-117-x.

2007.

objavljeno

Podaci o matičnoj publikaciji

Britvec, Mihaela ; Škvorc, Željko

Zagreb: Hrvatsko Botaničko Društvo

978-953-99774-1-0

Podaci o skupu

2. Hrvatski botanički kongres s međunarodnim sudjelovanjem

poster

20.09.2007-22.09.2007

Zagreb, Hrvatska

Povezanost rada

Biologija