engleski

HuIFN-α N3 affect the replication of avian influenza (H5N2) virus in the embryonated chicken eggs

Avian influenza (AI) H5N1 virus, the extremely contagious strain, can be deadly to domesticated poultry. Without » ; jumping« ; the species barrier it can seldom cause infections in humans. Such infection coincided with devastating epidemics in poultry farms in Asian countries, with a reported mortality rate approaching 100%. Closely related to this highly pathogenic avian influence (HPAI) is strain H5N2, being antigenically similar to H5N1 although distinguishable from an influenza H5N1 virus isolated from human in Hong Kong. Nevertheless phylogenetic analysis of the haemagglutinin (HA) genes showed that the highly pathogenic H5N2 virus clustered with the human H5N1 virus isolated in Hong Kong, even up to now, there were no available data of the transmission of this virus from bird to human. The presented experiments were aimed to find out if the low doses of human interferon HuIFN-α N3 affect the replication of the AI (H5N2) virus in the embryonated chicken eggs and through this the survival of infected embryos. The obtained results were evaluated on the basis of the EID50 value: 10 -4.249/0.1 ml, and where the following: VIRUS 10 – 4 + HuIFN-α N3 - SIMULTANEUSLY ultimately shows the effectiveness of low doses (150 I.U.) of IFN giving 100% of embryo survival and 100% of HA inhibition. VIRUS 10 – 4 + HuIFN-α N3 AFTER 2h only the high doses (15.000) are in some way effective (33.3% of survival) HuIFN-α N3+VIRUS 10 – 4 AFTER 2h are probably more important, because the pre-treatment with low doses and infection after 2 hours resulted in decrease of HA as well as 66.67% of embryo survival. This is probably connected with the neuraminidase (NA) inactivation. The further experiments will show us, how to optimize the effectiveness of HuIFN-α N3 toward the resistance of AI (H5N2) virus with different effectors like different l-amino acids.

avian influenza (H5N1); avian influenza (H5N2); HuIFNα N3; chicken embryo; infection; haemagglutination; embryo survival

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