The influence of -2518 A/G MCP-1 promoter polymorphism on homocysteine concentrations in patients with coronary artery disease (CROSBI ID 529658)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Gršković, Branka ; Pašalić, Daria ; Marinković, Natalija ; Ferenčak, Goran ; Stavljenić Rukavina, Ana
engleski
The influence of -2518 A/G MCP-1 promoter polymorphism on homocysteine concentrations in patients with coronary artery disease
Background. Monocyte chemoattractant protein (MCP)-1 is the key chemokine in the process of atherosclerotic vascular inflammation. MCP-1 and its receptor CCR2 have already been proposed as candidate genes for studies of genetic markers in coronary artery disease (CAD). Methods. The identification of the promoter polymorphism -2518 A/G was carried out using PCR, followed by a restriction fragment length polymorphism (RFLP) assay, using a PvuII site, which is introduced by the presence of the G nucleotide. Genotyping was performed in patients with (+) and without (-) angiographically confirmed CAD. Serum concentrations of CRP were determined by immunonephelometry, and homocysteine using fluorescence polarisation immunoassay (FPIA). Results. Statistically significant diference was not observed between the absolute frequencies of MCP-1 -2518 A/G gene polymorphism and CAD+ and CAD- subjects, p=0.913. The frequencies of MCP-1 -2518 AA, AG and GG genotypes were 69, 63 and 7 in CAD+ group, and 38, 35 and 5 in CAD- group, respectively. Statistically significant difference was found for median homocysteine concentrations between the MCP-1 genotypes in CAD- group, p=0.035. Median CRP concentrations were not statistically significant in both groups studied. After the corrections for sex and gender, statistically significant difference was found for median homocysteine concentrations in CAD+ (p=0.013) and CAD- (p=0.04) groups. Median CRP concentrations were not significantly different after the corrections for sex and gender in both groups studied. Conclusion. Homocysteine concentrations are significantly correlated with MCP-1 gene variants which confirm the importance of this polymorphism in genetics of CAD.
chemoattractant protein (MCP)-; polymorphism; coronary artery disease
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Podaci o prilogu
232-x.
2007.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Clinical chemistry and laboratory medicine
Gerard Siest
Amsterdam: Walter de Gruyter
1434-6621
Podaci o skupu
EUROMEDLAB Amsterdam 2007 17th IFCC-FESCC European Congress of Clinical Chemistry and Laboratory Medicine 60th National Congress of the Netherlands Society for Clinical Chemistry and Laboratory
poster
03.06.2007-07.06.2007
Amsterdam, Nizozemska
Povezanost rada
Javno zdravstvo i zdravstvena zaštita