engleski

The influence of -2518 A/G MCP-1 promoter polymorphism on homocysteine concentrations in patients with coronary artery disease

Background. Monocyte chemoattractant protein (MCP)-1 is the key chemokine in the process of atherosclerotic vascular inflammation. MCP-1 and its receptor CCR2 have already been proposed as candidate genes for studies of genetic markers in coronary artery disease (CAD). Methods. The identification of the promoter polymorphism -2518 A/G was carried out using PCR, followed by a restriction fragment length polymorphism (RFLP) assay, using a PvuII site, which is introduced by the presence of the G nucleotide. Genotyping was performed in patients with (+) and without (-) angiographically confirmed CAD. Serum concentrations of CRP were determined by immunonephelometry, and homocysteine using fluorescence polarisation immunoassay (FPIA). Results. Statistically significant diference was not observed between the absolute frequencies of MCP-1 -2518 A/G gene polymorphism and CAD+ and CAD- subjects, p=0.913. The frequencies of MCP-1 -2518 AA, AG and GG genotypes were 69, 63 and 7 in CAD+ group, and 38, 35 and 5 in CAD- group, respectively. Statistically significant difference was found for median homocysteine concentrations between the MCP-1 genotypes in CAD- group, p=0.035. Median CRP concentrations were not statistically significant in both groups studied. After the corrections for sex and gender, statistically significant difference was found for median homocysteine concentrations in CAD+ (p=0.013) and CAD- (p=0.04) groups. Median CRP concentrations were not significantly different after the corrections for sex and gender in both groups studied. Conclusion. Homocysteine concentrations are significantly correlated with MCP-1 gene variants which confirm the importance of this polymorphism in genetics of CAD.

chemoattractant protein (MCP)-; polymorphism; coronary artery disease

nije evidentirano