Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay (CROSBI ID 133473)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Želježić, Davor ; Domijan, Ana-Marija ; Peraica, Maja DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay // Brazilian Journal of Medical and Biological Research, 39 (2006), 12; 1563-1568-x

Podaci o odgovornosti

Želježić, Davor ; Domijan, Ana-Marija ; Peraica, Maja

engleski

DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay

There are few studies of ochratoxin A (OTA) genotoxicity in experimental animals and the results obtained with cell cultures are inconsistent, although the carcinogenic potential of OTA for the kidney of experimental animals has been well established. We studied the genotoxic potential of OTA in the kidney of adult female Wistar rats (5 in group) treated intraperitoneally with OTA (0.5 mg kg body weight-1 day-1 for 7, 14, and 21 days) measuring DNA mobility in agarose gel stained with ethidium-bromide using standard alkaline single-cell gel electrophoresis (comet assay). OTA concentrations in plasma and kidney homogenates in 7, 14, and 21 days treated animals were 4.86 &#177; ; 0.53, 7.52 &#177; ; 3.32, 7.85 &#177; ; 2.24 (mg/ml), and 0.87 &#177; ; 0.09, 0.99 &#177; ; 0.06, 1.09 &#177; ; 0.15 (mg/g), respectively. In all OTA-treated groups, the tail length, tail intensity, and tail moment in kidney tissue were significantly higher than in controls (P < 0.05). The tail length and tail moment were higher after 14 days than after 7 days of treatment (P < 0.05), and still higher after 21 days (P < 0.05). The highest tail intensity was observed in animals treated for 21 days, and it differed significantly from animals treated for 7 and 14 days (P < 0.05). OTA concentrations in plasma and kidney tissue increased steadily and OTA concentration in kidney tissue strongly correlated with tail intensity and tail moment values. These results confirm the genotoxic potential of OTA, and show that the severity of DNA lesions in kidney correlates with OTA concentration.

comet assay; DNA adducts; experimental animals; mycotoxin

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

39 (12)

2006.

1563-1568-x

objavljeno

0100-879X

Povezanost rada

Temeljne medicinske znanosti, Biologija

Indeksiranost