engleski

Differentiation in rat embryo experimental teratomas

9-days old Fischer rat embryos consisting of three germ-layers were cultivated using a modified organ culture method in a chemically defined serum-free medium and grafted under the kidney capsule of isogeneic animals for 14 days to obtain well developed experimental teratomas. The purpose of this experiment was to discover the influence of apotransferrin upon differentiation of a wider variety of tissues than those that usually develop in teratoma-like explants after in vitro culture only. It seems that apotransferrin alone enhanced overall differentiation process in experimental teratomas obtained from cultivated embryos.

experimental teratomas; embryo; culture; rat

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