engleski

In vivo EPR detection of spin-labelled ovalbumin applied subcutaneously in mice

Ovalbumin (OVA) is a glycoprotein often used as a model antigen of low immunogenicity in assessment of novel adjuvants. Liposomes are phospholipid vesicles, which are used as drug delivery systems and are known as immunological adjuvants. In our previous studies we have investigated effect of liposomes with OVA as antigen on the immune reaction in mice1. Interaction of OVA with liposom bilayers was also studied using EPR spectroscopy2. The aim of this study was to investigate our model antigen OVA as well as OVA entrapped in liposomes in vivo and ex vivo by electron paramagnetic resonance (EPR) spectroscopy in order to establish the distribution and pharmacokinetics of OVA (free or entrapped) within the body after subcutaneous (s.c.) application in mice. For this purpose the OVA was labelled with 4-maleimido-2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO-maleimide), covalently attached to the SH group of cystein within the protein.

in vivo EPR; liposomes; spin-labelled ovalbumin

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