engleski

Detection and typing of human papillomaviruses in cervical scrapes of adolescent women in Zagreb city area

Retrospective examination between adolescent women in Zagreb with cytomorphologically abnormal smears. Consensus and specific primers were used in the polymerase chain reaction (PCR) to detect the most common types of HPV:6. 11. 16. 18. 31 and 33. Out of 380 specimens, 165 (43%) contained one or more HPV types. Beside low-risk HPV 6/11 (26%) the most frequently observed types were high-risk HPV types 16 (20%) and 31 (18%). The presence of different HPV types significantly increased from 36% to 61% along with the severity of cervical intraepithelial neoplasia (from CIN I to CIN III). High-risk HPV type 16: increased from CIN I to III, other HPV types 18, 31 and 33 increased only from CIN I to II while low-risk HPV types 6 and 11 decreased from CIN I to III. The progression to high-grade CIN was strongly associated with high and moderate-risk HPV types while only a few progressions were associated with the presence of low-risk HPV types. The presence of HPV DNA significantly increased from 36% to 61% along with the severity of the cervical intraepithelial neoplasia (CIN I-III). HPV type 6/11 was strongly associated with CIN I (34%), HPV type 31 with CIN II (23%), and HPV type 16 with CIN III (50%). Zhe results of this study indicate a high prevalence of conventional high-risk HPV types among adolescent women in the Zagreb city area. There was a correlation between the distribution of HPV infections and the severity of cervical intraepithelial neoplasia.

HPV; Zagreb

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