engleski

The Use of Convective Interaction Media for Analysis of Long-Nosed Viper Venom

Confirmed also in this study, Vipera ammodytes ammodytes venom samples with higher content of ammodytoxins (Atxs), basic neurotoxic phospholipases A2, are more lethal, exposing Atxs as one of the major toxic components in the long-nosed viper venom. In addition, we recently correlated the ability of the venom to produce highly protective antiserum in rabbits with the amount of Atxs in the venom. Here, we developed a rapid, robust and highly reproducible chromatographic method based on HPLC separation on Convective Interaction Media to assess the quantity of Atxs in particular venom sample in order to predict its potential to induce highly-protective antiserum in immunized animals.

Vipera ammodytes ammodytes; ammodytoxins; Convective Interaction Media; antivenom production; long-nosed viper

Special Issue: Analysis of Polar Compounds