engleski

C-series polysialogangliosides are expressed on stellate neurons of adult human cerebellum

Until now 'c-series' polysialogangliosides were known to exist in human brain only during development and in some pathological conditions like Alzheimer's disease. Using thin-layer chromatography (TLC) and immunostainng with Q211 antibody (TLC-overlay techniwue) we have analysed 'c-series gangliosides in four human cerebella (age 20, 47, 52 and 54 years). Four distinct ganglioside bands, most probably corresponding to GT1c, GQ1c, GP1c and GH1c were found to exist in the analysed brain, which is convincing demonstration of the existence of c-series" gangliosides in normal adult human brain. Immunohistochemical analysis was performed to locate polysialogangliosides in the analysed tissu. Q211 antibody was found to bind specifically to a single subpopulation of neurons in the molecular layer of adult cerebellum. According to their position and morphology these cells correspond to stellate neurons.

gangliosides; human cerebellum; Q211 antibody; c-series gangliosides

nije evidentirano