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Pregled bibliografske jedinice broj: 300532

Preliminary crystallographic study of Streptomyces coelicolor single-stranded DNA-binding protein


Štefanić, Zoran; Vujaklija, Dušica; Andrišić, Luka; Mikleušević, Goran; Andrejašič, Miha; Turk, Dušan; Luić, Marija
Preliminary crystallographic study of Streptomyces coelicolor single-stranded DNA-binding protein // Croatica chemica acta, 80 (2007), 1; 35-39 (međunarodna recenzija, članak, znanstveni)


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Naslov
Preliminary crystallographic study of Streptomyces coelicolor single-stranded DNA-binding protein

Autori
Štefanić, Zoran ; Vujaklija, Dušica ; Andrišić, Luka ; Mikleušević, Goran ; Andrejašič, Miha ; Turk, Dušan ; Luić, Marija

Izvornik
Croatica chemica acta (0011-1643) 80 (2007), 1; 35-39

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
single-stranded DNA-binding protein ; SSB purification ; Streptomyces coelicolor ; crystallisation
(Single-stranded DNA-binding protein ; SSB purification ; Streptomyces coelicolor ; crystallisation)

Sažetak
Single-stranded DNA-binding proteins (SSBs) play a crucial role in DNA processing such as replication, repair and recombination in all organisms, from bacteria to human. Streptomyces coelicolor ssb gene was overexpressed in a heterologous host, Escherichia coli NM522. 15 mg of purified protein from 1 dm3 of culture was obtained in one-step procedure applying Ni2+ chelating chromatography. Among bacterial SSBs with the solved crystal structure, the S. coelicolor SSB displayed significant sequence similarity with those from Mycobacterium tuberculosis and Mycobacterium smegmatis, slow growing bacteria with a high GC content. Moreover, conserved amino acid region that forms additional β strand in mycobacterial SSBs was also found in S. coelicolor SSB. The full-length protein readily crystallises in space group I222 or I212121 with unit-cell parameters a = 100.8, b = 102.1, c = 164.2 Å ; . The asymmetric unit is expected to contain four monomers with solvent content of 52-55 %.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija



POVEZANOST RADA


Projekt / tema
098-0982913-2877 - Temeljna molekularno-biološka istraživanja streptomiceta (Dušica Vujaklija, )
098-1191344-2943 - Protein-ligand međudjelovanja na atomnoj razini (Marija Luić, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Citiraj ovu publikaciju

Štefanić, Zoran; Vujaklija, Dušica; Andrišić, Luka; Mikleušević, Goran; Andrejašič, Miha; Turk, Dušan; Luić, Marija
Preliminary crystallographic study of Streptomyces coelicolor single-stranded DNA-binding protein // Croatica chemica acta, 80 (2007), 1; 35-39 (međunarodna recenzija, članak, znanstveni)
Štefanić, Z., Vujaklija, D., Andrišić, L., Mikleušević, G., Andrejašič, M., Turk, D. & Luić, M. (2007) Preliminary crystallographic study of Streptomyces coelicolor single-stranded DNA-binding protein. Croatica chemica acta, 80 (1), 35-39.
@article{article, year = {2007}, pages = {35-39}, keywords = {Single-stranded DNA-binding protein, SSB purification, Streptomyces coelicolor, crystallisation}, journal = {Croatica chemica acta}, volume = {80}, number = {1}, issn = {0011-1643}, title = {Preliminary crystallographic study of Streptomyces coelicolor single-stranded DNA-binding protein}, keyword = {Single-stranded DNA-binding protein, SSB purification, Streptomyces coelicolor, crystallisation} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • Chemical Abstracts