engleski

CD38 antigen on bronchoalveolar T lymphocytes correlate with alveolitis activity in interstitial lung diseases (ILD)

Recent data suggest that CD38 may be involved in lymphocyte activation and proliferation. The aim of this study was to clarify the role of CD38 in alveolitis pathogenesis. CD38 expression on T cells in bronchoalveolar lavate (BAL) of patients with ILD was assessed by double-staining with CD4/CD38 and CD8/CD38 mAbbs and the data were compared with the same parameters mesured in relevant diseases free subjects (C=8). The ILD samples were obtained from: active sarcoidosis (SA=16), inactive sarcoidosis (SI=15) and idiopathic pulmonary fibrosis (IPF=8). There was variable, but often low number of CD38 cells in control BAL samples. Proportions of CD4, CD38 and CD4CD38 were highly elevated only in SA as compared to SI and IPF, whereas CD8CD38 were generally low. T subset analysis revealed that CD38 was in C predominantly expressed on CD8, whereas in ILD equally on CD4 and CD8 lymphocytes. In three years follow up study eight patients showed CD4CD38 variations depending on the disease activity. As local CD4CD38 expansion correlates with the onset and disease progression, we assume that in SA an intensive recruitment of activated cells is taking place through autocrine fashion by CD38 pathway. Our results indicate that CD38 surface antigen may play a relevant role in SA pathogenesis and could serve as an indicator of alveolitis activity.

CD38; BAL; ILD; Flow cytometry

nije evidentirano