engleski

Correlation between Severity of Cervical Intraepithelial Neoplasia Disorders and Frequency of Low- and High-risk HPV Genotype Distribution in Patients from Eastern Croatia

Human papillomavirus (HPV) genotyping have shown to be valuable toll for detection of HPV infection in cervical precancerous lesions, cervical cancer and in different anogenital disorders like condyloma accuminata and cervicitis. In addition to Papanicolaou staining, cervical swab samples from 353 women were subsequently tested by PCR- ELISA method. Following isolation DNA quality control was performed using PCR target specific β -globin primers. In 11 (3.11%) out of 353 DNA samples β globin PCR reaction was inhibited. PCR mixture containing consensus primers (MY09 and MY11) and dNTPs tagged with digoxigenin molecule was used for amplification of viral L1 gene. Afterwards, PCR products were tested in ELISA assay using biotin-labeled hybridization probes specific for L1 gene of different low-risk (LR) and high-risk (HR) HPV types. L1 negative DNA samples were additionally tested for E6/E7 viral genes using target specific consensus primers. Out of 336 completely analyzed samples HPV was determined in 136 (40.47%) specimens. Frequency of LR- and HR-HPV types was compared with Pearson's Chi2-test or by Fisher exact test whenever appropriate. Significance was accepted at p<0.05. No statistically significant difference was observed between LR- and HR-HPV frequency in CIN- 1, -2, or -3, even though results indicate that HPV frequency growth follows severity of CINs. However, when comparing frequency of low- and high risk HPV types independently, significant difference in HPV frequency was determined between CIN -1, -2, -3 groups and condyloma accuminata group of samples. The LR-HPV genotypes were found to be more frequent in CIN-1 (χ 2=6.106, p=0.013, OR=3.91 (95% CI) 1.16-16.82), CIN-2 (χ 2=8.992, p=0.003, OR=4.87 (95% CI) 1.51-20.73) and CIN-3 (χ 2=10.010, p=0.002, OR=5.1 (95% CI) 1.63- 22.09) when compared to condyloma accuminata. Conversely, frequency of HR-HPV genotypes was found to be significantly lower only in CIN I when compared to condyloma accuminata group (χ 2=6.486, p=0.011, OR=0.47 (95% CI) 0.24-0.88).

LR-HPV ; HR-HPV ; CIN ; Eastern Croatia

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