engleski

Epitope Mapping of Macrolide Antibiotics to Bovine Serum Albumin by Saturation Transfer Difference NMR Spectroscopy

Saturation transfer difference NMR spectroscopy was employed to characterize epitopes of macrolide antibiotics, azithromycin, oleandomycin and telithromycin binding to bovine serum albumine. The structural parts of azithromycin and oleandomycin in intimate contact to bovine serum albumine were found to be similar while those of telithromycin showed similarities but also some differences. Those were mostly due to different structural elements of antibiotics that interact with the protein especially alkyl-heteroaryl side chain in telithromycin and cladinose and desosamine sugars in azithromycin and oleandomycin. The epitope maps as determined in this study can help in better understanding of the overall bioactivity of macrolides.

STD NMR spectroscopy; macrolide antibiotics; albumine; epitope mapping

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