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Pregled bibliografske jedinice broj: 296525

Early Toxic Effects of Fumonisin B1 in Rat Liver


Domijan, Ana-Marija; Želježić, Davor; Peraica, Maja; Kovačević, Goran; Gregorović, Gordana; Krstanac, Željko; Horvatin, Karlo; Kalafatić, Mirjana
Early Toxic Effects of Fumonisin B1 in Rat Liver // Human and Experimental Toxicology, 27 (2008), 12; 895-900 doi:10.1177/0960327108100418 (međunarodna recenzija, članak, znanstveni)


Naslov
Early Toxic Effects of Fumonisin B1 in Rat Liver

Autori
Domijan, Ana-Marija ; Želježić, Davor ; Peraica, Maja ; Kovačević, Goran ; Gregorović, Gordana ; Krstanac, Željko ; Horvatin, Karlo ; Kalafatić, Mirjana

Izvornik
Human and Experimental Toxicology (0960-3271) 27 (2008), 12; 895-900

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Apoptosis; comet assay; glutathione; malondialdehyde; mycotoxin; necrosis

Sažetak
Mycotoxin fumonisin B1 (FB1) is hepatotoxic and carcinogenic in experimental animals. It is known that long-term exposure of experimental animals to FB1 causes apoptosis and lipid peroxidation. In this study, male adult Wistar rats were treated with single FB1 doses (5, 50, and 500 µ ; g/kg b.w.) and sacrificed 4, 24, and 48 hours after treatment. Parameters of oxidative stress, histopathological changes, and DNA damage were monitored in the liver of treated and control animals. Parameters of oxidative stress were not affected by such treatment. A significant increase in apoptotic cells appeared in animals when 5 µ ; ; g/kg b.w. dose was given and sacrificed after 24 hours with further increase at higher doses. In contrast to the number of mitotic figures and karyomegaly seen mostly at lower FB1 doses, necrosis was the prominent feature at higher doses. Significant increase in liver cells DNA mobility was observed 48 hours following treatment with 50 and 500 µ ; g/kg b.w. as compared to control (tail length 15.2 ± ; 0.3, 16.4 ± ; 0.5, and 13.5 ± ; 0.1 µ ; g/kg b.w., respectively). Tail intensity appeared to be more sensitive parameter for detecting DNA damage even at 5 µ ; g/kg b.w. after 48 hours (1.69 ± ; 0.27% DNA ; control 0.59 ± ; 0.11% DNA). This study proved that FB1-induced DNA damage is time- and dose-dependent, and that it could be caused in Wistar rats by a single dose.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekt / tema
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Vilena Kašuba, )
022-0222148-2142 - Toksični učinci mikotoksina na ljude i životinje (Maja Peraica, )
119-0000000-3171 - Razvojna i populacijska biologija velikih morskih kralježnjaka u Jadranskom moru (Gordana Lacković-Venturin, )
119-1193080-1214 - Molekularna filogenija, evolucija i simbioza slatkovodnih beskralježnjaka (Mirjana Kalafatić, )
119-1193080-3171 - Razvojna i populacijska biologija velikih morskih kralježnjaka u Jadranskom moru (Gordana Lacković-Venturin, )

Ustanove
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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