engleski

Determination of the Veterinary Antibiotics Pollution in Soil from Agricultural Sources

Emission of veterinary antibiotics has emerged as an environmental problem and a lack of knowledge concerning this kind of pollution requests further investigation. The antibiotics primarily used in animal medicine are tetracyclines, sulfonamides, β -lactames and fluoroquinolones. Antibiotics reach agricultural soils directly through livestock or indirectly through the use of manure and sewage sludge as fertilizers. The aim of this work was to optimise the conditions for ultrasonic solvent extraction (USE) of the most commonly used antibiotics in Croatian veterinary practice: sulfamethazine, sulfadiazine, sulfaguanidine, norfloxacine, oxytetracyclin, penicillinG/procaine and trimethoprim from soil samples. Sonication provides a more efficient contact between the solid and solvent than classical extraction procedures, resulting in a greater recovery of analyte. The extracted pesticides were identified using following thin-layer chromatography (TLC) system: HPTLC CN F254 chromatography plates as stationary phase, and 0.05 M H2C2O4:methanol=0.80:0.20 as mobile phase. Quantification was performed using 3CCD colour video camera.

veterinary antibiotics; soil; ultrasonic solvent extraction; TLC

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