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Pregled bibliografske jedinice broj: 292030

Synthesis and Characterization of a C(6) Nucleoside Analogue for the in vivo Imaging of the Gene Expression of Herpes Simplex Virus Type-1 Thymidine Kinase (HSV1 TK)


Johayem, Anass; Raić-Malić, Silvana; Lazzati, Katia; Schubiger, Pius A.; Scapozza, Leonardo; Ametamey, Simon M.
Synthesis and Characterization of a C(6) Nucleoside Analogue for the in vivo Imaging of the Gene Expression of Herpes Simplex Virus Type-1 Thymidine Kinase (HSV1 TK) // Chemistry and Biodiversity, 3 (2006), 3; 274-283 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 292030 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Synthesis and Characterization of a C(6) Nucleoside Analogue for the in vivo Imaging of the Gene Expression of Herpes Simplex Virus Type-1 Thymidine Kinase (HSV1 TK)

Autori
Johayem, Anass ; Raić-Malić, Silvana ; Lazzati, Katia ; Schubiger, Pius A. ; Scapozza, Leonardo ; Ametamey, Simon M.

Izvornik
Chemistry and Biodiversity (1612-1872) 3 (2006), 3; 274-283

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
pyrimidine-nucleoside analogues; 18F-radiolabelling; HSV-1 TK; phosphorylation; binding affinity

Sažetak
The synthesis and biological evaluation of "6-(1, 3-dihydroxyisobutyl)thymine" (DHBT ; 1), which corresponds to 6-[3-hydroxy-2-(hydroxymethyl)propyl]-5-methylpyrimidine-2, 4(1H, 3H)-dione, is reported. DHBT (1) was designed as a new substrate for herpes simplex virus type-1 thymidine kinase (HSV1 TK). The compound was found to be exclusively phosphorylated by HSV1 TK, and to exhibit good binding affinity (Ki = 35.3 &plusmn ; ; 1.3 μ M). Cell-proliferation assays with HSV1-TK-transduced human osteosarcoma cells (143B-TK+-HSV1-WT) and with both human-thymidine-kinase-1-negative (143B-TK– ) and non-transduced parental (MG-63) cells indicate that 1 is less cytotoxic than the standard drug Ganciclovir. Thus, DHBT (1) represents a promising precursor of a nontoxic reporter probe for the monitoring of HSV1 TK gene expression by means of positron-emission tomography (PET).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
125-0982464-2925 - Razvoj i primjena novih molekula u pozitron-emisijskoj tomografiji (PET) (Raić-Malić, Silvana, MZOS ) ( POIROT)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Avatar Url Silvana Raić-Malić (autor)


Citiraj ovu publikaciju:

Johayem, Anass; Raić-Malić, Silvana; Lazzati, Katia; Schubiger, Pius A.; Scapozza, Leonardo; Ametamey, Simon M.
Synthesis and Characterization of a C(6) Nucleoside Analogue for the in vivo Imaging of the Gene Expression of Herpes Simplex Virus Type-1 Thymidine Kinase (HSV1 TK) // Chemistry and Biodiversity, 3 (2006), 3; 274-283 (međunarodna recenzija, članak, znanstveni)
Johayem, A., Raić-Malić, S., Lazzati, K., Schubiger, P., Scapozza, L. & Ametamey, S. (2006) Synthesis and Characterization of a C(6) Nucleoside Analogue for the in vivo Imaging of the Gene Expression of Herpes Simplex Virus Type-1 Thymidine Kinase (HSV1 TK). Chemistry and Biodiversity, 3 (3), 274-283.
@article{article, year = {2006}, pages = {274-283}, keywords = {pyrimidine-nucleoside analogues, 18F-radiolabelling, HSV-1 TK, phosphorylation, binding affinity}, journal = {Chemistry and Biodiversity}, volume = {3}, number = {3}, issn = {1612-1872}, title = {Synthesis and Characterization of a C(6) Nucleoside Analogue for the in vivo Imaging of the Gene Expression of Herpes Simplex Virus Type-1 Thymidine Kinase (HSV1 TK)}, keyword = {pyrimidine-nucleoside analogues, 18F-radiolabelling, HSV-1 TK, phosphorylation, binding affinity} }
@article{article, year = {2006}, pages = {274-283}, keywords = {pyrimidine-nucleoside analogues, 18F-radiolabelling, HSV-1 TK, phosphorylation, binding affinity}, journal = {Chemistry and Biodiversity}, volume = {3}, number = {3}, issn = {1612-1872}, title = {Synthesis and Characterization of a C(6) Nucleoside Analogue for the in vivo Imaging of the Gene Expression of Herpes Simplex Virus Type-1 Thymidine Kinase (HSV1 TK)}, keyword = {pyrimidine-nucleoside analogues, 18F-radiolabelling, HSV-1 TK, phosphorylation, binding affinity} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka::


  • Chemical Abstracts
  • Excerpta Medica
  • Index Medicus





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