engleski

Toxicity of metals on the growth of Raphidocelis subcapitata and Chlorella kessleri using microplate bioassays

Toxicities of CuCl_2 • 2H_2O, CoCl_2 • 6H_2O, HgCl_2 and FeCl_3 • 6H_2O were tested using algal growth inhibition bioassays on two freshwater green algae Raphidocelis subcapitata (=Selenastrum capricornutum) and Chlorella kessleri. The aim was to compare the algal growth inhibition EC50s obtained from the toxicity testing of four metal compounds on microplates with the standard flask assay, as well as to compare the sensitivity of tested algae to selected metal compounds. The results showed that mercury was the most toxic element for both algae. The toxicity hierarchy for both algae, according to the mean values from both methods was: Hg>Cu>Co>Fe. C. kessleri was more sensitive (0.11 – 8 mg L^-1, with average value of 1.777 mg L^-1) than R. subcapitata (0.33 – 9.4 mg L^-1 ; average 2.059 mg L^-1) to tested metal compounds. Algal bioassay in microplates gave comparable results to standard flask bioassay for tested algae and can be recommended for the routine toxicity testing.

Chlorella kessleri; EC50; growth bioassay; growth rate; metals; Raphidocelis subcapitata

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