engleski

Cathepsin D content in endometrial carcinoma

The aim of this study was to determine the concentration of cathepsin D in normal uterine tissue and endometrial carcinoma (30 matched pairs), and to assess the relationship between Cathepsin D content and clinical and pathohistological prognostical parameters in uterine carcinomas. The samples representing matched pairs were obtained from the tumour and adjacent normal tissue. The cathepsin D content was measured in cytosol of homogenated samples, using a commercially available solid-phase two-site immunoradiometric assay (CIS Bio International, Gif-sur-Yvette, France). The total protein in cytosol was determined by Bradford-s method. We found that the concentrations of cathepsin D were significantly higher in tumours (35,0ą19,02 pmol/mg protein) than in normal tissue (11,87ą5,21 pmol/mg protein) p< 0.001 with a tumour to normal tissue ratio of 2,95. The concentrations of cathepsin D did not seem to relate to clinical stage (I versus II and III), myometrial invasion, tumour grade, histological type of tumour, lymphovascular invasion, and patients age.

Cathepsin D; endometrial carcinoma

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