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Pregled bibliografske jedinice broj: 289746

Rapid Extraction of Human DNA containing humic acid


Sutlović, Davorka; Definis Gojanović, Marija; Anđelinović, Šimun
Rapid Extraction of Human DNA containing humic acid // Croatica Chemica Acta, 80 (2007), 1; 117-120 (međunarodna recenzija, članak, znanstveni)


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Naslov
Rapid Extraction of Human DNA containing humic acid

Autori
Sutlović, Davorka ; Definis Gojanović, Marija ; Anđelinović, Šimun

Izvornik
Croatica Chemica Acta (0011-1643) 80 (2007), 1; 117-120

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
human DNA; extraction; humic acid; DNA inhibition

Sažetak
The identification process of dead bodies or human remains is nowdays conducted in numerous fields of forensic science, archeology and other juditial cases. A particular problem is the isolation and DNA typing of human remains found in mass graves, due to the degradation process, as well as post mortal DNA contamination with bacteria, fungi, humic acids, metals etc. In this study we investigated the influence of humic acid (HA) on the DNA extraction and typing. We reported that the humic acid if present in the amplification reaction mix inhibited the DNA amplificatio, but the addition of 50 mg PVPP to the reaction mixture appeared to be optimum in overcoming that inhibition.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
141-2160800-0333 - DNA analiza ranosrednjovjekovne populacije s područja južne Hrvatske
216-2160800-0655 - Metali u kosturima iz starohrvatskih grobova u Kliškoj i Bribirskoj županiji (Sutlović, Davorka, MZOS ) ( POIROT)
216-2160800-0799 - Antropološka analiza kostura ranosrednjovjekovne populacije iz južne hrvatske (Definis-Gojanović, Marija, MZOS ) ( POIROT)

Ustanove:
Medicinski fakultet, Split

Poveznice na cjeloviti tekst rada:

Hrčak

Citiraj ovu publikaciju:

Sutlović, Davorka; Definis Gojanović, Marija; Anđelinović, Šimun
Rapid Extraction of Human DNA containing humic acid // Croatica Chemica Acta, 80 (2007), 1; 117-120 (međunarodna recenzija, članak, znanstveni)
Sutlović, D., Definis Gojanović, M. & Anđelinović, Š. (2007) Rapid Extraction of Human DNA containing humic acid. Croatica Chemica Acta, 80 (1), 117-120.
@article{article, author = {Sutlovi\'{c}, Davorka and Definis Gojanovi\'{c}, Marija and An\djelinovi\'{c}, \v{S}imun}, year = {2007}, pages = {117-120}, keywords = {human DNA, extraction, humic acid, DNA inhibition}, journal = {Croatica Chemica Acta}, volume = {80}, number = {1}, issn = {0011-1643}, title = {Rapid Extraction of Human DNA containing humic acid}, keyword = {human DNA, extraction, humic acid, DNA inhibition} }
@article{article, author = {Sutlovi\'{c}, Davorka and Definis Gojanovi\'{c}, Marija and An\djelinovi\'{c}, \v{S}imun}, year = {2007}, pages = {117-120}, keywords = {human DNA, extraction, humic acid, DNA inhibition}, journal = {Croatica Chemica Acta}, volume = {80}, number = {1}, issn = {0011-1643}, title = {Rapid Extraction of Human DNA containing humic acid}, keyword = {human DNA, extraction, humic acid, DNA inhibition} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


Uključenost u ostale bibliografske baze podataka::


  • Chemical Abstracts





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