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A novel method for flow injection analysis of total antioxidant capacity using enzymatically produced ABTS and biamperometric detector containing interdigitated electrode

Application of interdigitated array microelectrodes as electrochemical sensor for determination of blood serum antioxidant capacity is reported. Electrochemical measurements with interdigitated electrodes (IDE) were studied in both, stationary solutions and in flow system. The method is based on the biamperometric measurements using ABTS+| ABTS redox couple in the phosphate buffer solution, pH = 7.40. During the analysis the ABTS radical cation was enzymatically produced by peroxidase in the tubular flow-through reactor. The performance of bioreactor was tested at different concentration of immobilized enzyme, ABTS and hydrogen peroxide. The influence of flow to the properly work of bioreactor was also studied. The results of antioxidant activity were determined using Trolox as a standard. The applied IDE detector accomplished good sensitivity of 0.3 nA/&micro ; M of Trolox and offered linear range between 20 &micro ; M to 500 &micro ; M of Trolox. The comparison of results (R= 0.9986) for antioxidant activity of blood serum between spectroscopic and FIA biamperometric measurements by interdigitated electrodes confirmed the applicability of the proposed method for determination of antioxidant capacity.

interdigitated electrodes; antioxidant; biamperometry; Trolox

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