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Pregled bibliografske jedinice broj: 287821

A novel method for flow injection analysis of total antioxidant capacity using enzymatically produced ABTS and biamperometric detector containing interdigitated electrode


Milardović, Stjepan; Kereković, Irena; Derrico, Romano; Rumenjak, Vlatko
A novel method for flow injection analysis of total antioxidant capacity using enzymatically produced ABTS and biamperometric detector containing interdigitated electrode // Talanta, 71 (2007), 1; 213-220 (međunarodna recenzija, članak, znanstveni)


Naslov
A novel method for flow injection analysis of total antioxidant capacity using enzymatically produced ABTS and biamperometric detector containing interdigitated electrode

Autori
Milardović, Stjepan ; Kereković, Irena ; Derrico, Romano ; Rumenjak, Vlatko

Izvornik
Talanta (0039-9140) 71 (2007), 1; 213-220

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Interdigitated electrodes; antioxidant; biamperometry; Trolox

Sažetak
Application of interdigitated array microelectrodes as electrochemical sensor for determination of blood serum antioxidant capacity is reported. Electrochemical measurements with interdigitated electrodes (IDE) were studied in both, stationary solutions and in flow system. The method is based on the biamperometric measurements using ABTS+| ABTS redox couple in the phosphate buffer solution, pH = 7.40. During the analysis the ABTS radical cation was enzymatically produced by peroxidase in the tubular flow-through reactor. The performance of bioreactor was tested at different concentration of immobilized enzyme, ABTS and hydrogen peroxide. The influence of flow to the properly work of bioreactor was also studied. The results of antioxidant activity were determined using Trolox as a standard. The applied IDE detector accomplished good sensitivity of 0.3 nA/&micro ; M of Trolox and offered linear range between 20 &micro ; M to 500 &micro ; M of Trolox. The comparison of results (R= 0.9986) for antioxidant activity of blood serum between spectroscopic and FIA biamperometric measurements by interdigitated electrodes confirmed the applicability of the proposed method for determination of antioxidant capacity.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
0125054

Ustanove
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE