DNA repair in Deinococcus radiodurans: from hundreds of fragments to the functional chromosomes (CROSBI ID 525469)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Zahradka, Ksenija ; Slade, Dea ; Bailone, Adriana ; Sommer, Suzanne ; Averbeck, Dietrich ; Petranović, Mirjana ; Lindner, Ariel ; Radman, Miroslav
engleski
DNA repair in Deinococcus radiodurans: from hundreds of fragments to the functional chromosomes
Bacterium Deinococcus radiodurans is one of the most radiation resistant organisms known. It can sustain extreme levels of ionizing radiation breaking its genome into hundreds of shorts fragments. Remarkably, in just couple of hours, these fragments are reassembled into functional chromosomes due to an efficient and precise DNA repair process. We found that this repair involves a special kind of “ extended synthesis-dependent strand annealing” (ESDSA) followed and completed by crossovers. In ESDSA, chromosomal fragments produced by radiation are used both as primers and templates for a massive synthesis of long single-strand extensions which then anneal thereby accurately connecting contiguous DNA fragments into long linear intermediates. These intermediates are finally matured by RecA-dependent homologous recombination into functional circular chromosomes that are patchworks of old (synthesized before radiation) and new (synthesized after radiation) double-stranded DNA blocks.
Deinococcus radiodurans; DNA repair; DNA synthesis; homologous recombination
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Podaci o prilogu
2006.
objavljeno
Podaci o matičnoj publikaciji
Current Trends in Biomedicine Workshop: Mechanism and biological consequences of recombinational DNA repair-mediated genome instability
Aguilera, A. ; Kanaar, R.
Baeza:
Podaci o skupu
Current Trends in Biomedicine: Mechanisms and biological consequences of recombinational DNA repair-mediated genome instability
poster
06.11.2006-08.11.2006
Baeza, Španjolska