engleski

AN ASSESSMENT OF BOVIPURE® AND SWIM UP SEPARATION OF BULL SPERMATOZOA FOR IN VITRO FERTILIZATION

The aim of our research was to examine the ability of density gradient separation BoviPure&#174; and swim up method on bull sperm separation and in vitro embryo production (IVP). Frozen/thawed semen from six Simmental bulls was pooled and treated using both methods. The sperm motility, concentration, membrane activity, membrane integrity and acrosomal status were evaluated and compared before and after sperm processing using BoviPure&#174; and swim up methods. A total of 641 oocytes were matured and fertilized in vitro and cultured in SOFaaBSA. The cleavage rates on Day 2, the total number of morulas (M) and blastocysts (Bl) on Day 7 and the numbers of hatched blastocysts (hBl) on Day 9 were recorded. Differential staining of the inner cell mass (ICM) and trophectoderm cells were performed on Day 7 blastocysts. The percentage of cleavage and the percentage of hatched embryos were similar for both methods, 77.25 &#177; 2.02% ; 14.88 &#177; 2.38% for BoviPure&#174; and 72.63 &#177; 3.98% ; 12.11 &#177; 0.69% for swim up. However, embryo production rate at Day 7 was significantly higher using BoviPure&#174; method (P<0.05) vs. swim up, 31.79 &#177; 0.71% and 21.91 &#177; 2.49%, respectively. Also, total cell number and embryo differential staining of day 7 blastocysts showed that BoviPure&#174; treated sperm displayed better quality embryos compared to swim up method (P<0.05). Therefore, our results indicate that BoviPure&#174; method has an enhanced capacity in sperm selection for in vitro embryo production when compared with swim up method. So, we concluded that BoviPure&#174; could be considered as a better alternative to swim up method for separating bull spermatozoa from frozen/thawed semen for IVP of bovine embryos

sperm; bovipure; swim up; in vitro production

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