engleski

Frequency of PON2 gene S311C polymorphism in patients with chronic renal failure

The paraoxonase (PON) gene family, located on the long arm of chromosome 7q21.3-22.1, includes three members (pon1, pon 2, pon 3) that code for enzymes involved in the protection against oxidative stress. Paraoxonase 2 (PON2) is ubiquitously expressed in nearly all human tissues and acts as cellular antioxidant. Due to the reduction of oxidative stress PON2 may also act as an antiatherogenic enzyme. Common polymorphism of pon2 gene related to the serine/cysteine substitution at position 311 (S311C) has been reported to be associated with the high risk of atherosclerosis. We determined S311C polymorphism of pon2 gene in patients with chronic renal failure undergoing haemodialysis (N=160) that have an increased risk of developing atherosclerosis, and compared obtained results with the control group (N=167). For S311C polymorphism detection we used standard procedure: Miller's method for DNA isolation from whole blood, and PCR-RFLP method for polymorphism analysis. The analysis of pon2 S311C gene polymorphism in patients with chronic renal failure showed following frequencies: 88 (0, 55) SS, 63 (0, 39) CS and 9 (0, 06) CC. In the control group we found rather similar frequencies: 83 (0, 50) SS, 80 (0, 48) CS and 4 (0, 02) CC. Allele frequencies in patients were 239 (0, 75) for S allele and 81 (0, 25) for C allele. In control group we found following allele frequency 246 (0, 74) for S allele and 88 (0, 26) for C allele. According to the statistical evaluation by Chi-square test we concluded that there was no significant difference in distribution of S311C genotype (P = 0, 139) and allele frequency (P = 0, 831) between patients with chronic renal failure and healthy controls.

S311C polymorphism; Chronic renal failure

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