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Relationship between Helicobacter pylori CagA and VacA serological status and bacterial density on gastric mucosa

Aim: to evaluate the effects of CagA and VacA seropositivity on Helicobacter pylori bacterial density in bioptic samples of gastric mucosa. Methods: study was done on 207 patients undergoing upper endoscopy because of dyspeptic complaints. All patients were Helicobacter pylori positive based on results of histology, serology, urea breath test and microbiology. In all patients serum antibodies to p 120 (CagA) and p 95 (VacA) proteins of bacteria Helicobacter pylori were assessed by using Western blot method (Westernblot, Euroimmun Medizinische Labordiagnostika AG, Lubeck). Histological data were analysed according updated Sydney classification with special attention to grade of Helicobacter pylori colonization. Statistical analysis was performed using  2 test (STATISTICA for Windows, Release 5.5 H i SPSS for Windows, Release 7.5). Results: the study population consisted of 91, 3 % (189/207) CagA antibodies positive and 75, 8 % (157/207) VacA antibodies positive patients. The distrubution of CagA and VacA positive and negative patients according to Helicobacter pylori bacterial load grade are presented in Table 1. Corpus Antrum Grade 1 Grade 2 Grade 3 Grade 1 Grade 2 Garde 3 CagA + 108 50 31 66 69 54 CagA neg. 13 2 3 9 4 5 VacA + 92 40 25 55 62 40 VacA neg. 30 12 8 20 11 19 Table 1. CagA and VacA serological status and bacterial density No statistically significant difference in grades of bacterial load was found between CagA positive and CagA negative patienets in korpus and antrum of gastric mucosa ( 2 = 2, 180, df 2, p<1 ;  2 = 1, 986, df 2, p<1). The same results were obtained for VacA postive and VacA negative patinets ( 2 = 0, 0515, df 2, p<1 ;  2 = 5, 634, df 2, p<0, 1). Conclusions: CagA and VacA seropositivity have no influence on bacterial load grade in gastric mucosa.

Helicobacter pylori; CagA; VacA; bacterial density

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