Rapid loss of Oct-4 expression and pluripotency in cultured embryonic stem cells derived from Non-obese diabetic mice (CROSBI ID 522963)
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Podaci o odgovornosti
Korolija, Marina ; Popović Hadžija, Marijana ; Hadžija, Mirko
engleski
Rapid loss of Oct-4 expression and pluripotency in cultured embryonic stem cells derived from Non-obese diabetic mice
The POU transcription factor Oct-4 has been characterized as a regulator of ES cell pluripotency. Therefore we have tested the ability of NOD mice ES cells to remain pluripotent in primary culture by monitoring Oct-4 expression over a five day period. Although the ES cells have been cultured on feeder layer prepared from primary embryonic fibroblasts, in culture media supplemented with leukemia inhibitory factor (in order to prevent differentiation), Oct-4 expression has been detectable by RT-PCR only during first three days of culturing. In contrast, ES cells derived from blastocysts of various mouse strains (Balb/C, C57Bl/GoZg and C3H) grown in identical culture conditions have retained Oct-4 expression throughout entire five day period. Consistent with that observation, morphology of NOD ES cells has confirmed loss of pluripotency by the fourth day of culturing (appearance of giant trophoblast cells and fibroblast-like cells), while ES cells of other tested strains have retained typical pluripotent, undifferentiated phenotype (small cells with large nuclei and minimal cytoplasm). Based on obtained results, we have concluded that early loss of Oct-4 expression and pluripotency (i.e. onset of differentiation) in cultured NOD mice blastocysts could be a limiting factor in establishment of ES cell line.
Oct-4; NOD; embryonic stem cells
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Podaci o prilogu
2006.
objavljeno
Podaci o matičnoj publikaciji
Podaci o skupu
EMBO/HHMI Central European Scientists Meeting
poster
15.06.2006-17.06.2006
Cavtat, Hrvatska