engleski

Influence of H. pylori infection and eradication on p53, c-erbB-2 and Ki-67 in gastric mucosa

ABSTRACT: AIM: To evaluate the expression of antigens c-erbB-2, p53, and Ki-67 in gastric biopsy, bacteria density and urease activity in two groups of patients with chronic gastritis separate on the basis of the success or failure of H. pylori eradication. METHODOLOGY: Sixty-five patients with chronic gastritis were split in two groups (n=45/20) related to response to the therapy. The gastric mucosa samples (Sydey system) were analyzed histological in both groups of patients before and after standard therapy (for eradicated, E group after one cycle ; for non-eradicated, NE group after three cycles) for H. pylori infection, urease activity, c-erbB-2, p53 and Ki-67 expression. Sera samples taken before and after treatment were also analyzed for specific antibody against H. pylori. RESULTS: The eradication of H. pylori in patients of E group was accompanied with significant lower colonization grades of bacteria, urease activity, proliferating rate, p53, and Ki-67 expression while c-erbB-2 expression was unchanged. In NE group, all parameters were the same before and after therapy with exception of p53, which was slightly higher on both locations. Strong expression of c-erbB-2 in corpus of NE group was determinate. Serum activity of specific antibodies against H. pylori was lower after the therapy in both groups of patients, but in eradicated group this change was much stronger that in non-eradicated. CONCLUSION: Long persisting infection is related with higher colonization grades of bacteria, urease activity, p53, c-erbB-2 and Ki-67 expression. Changes of those markers are connected with duration of infection and location where this changes were obtained.

Helicobacter pylori; gastric mucosa infection; eradication therapy; p53; c-erbB-2; Ki-67

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