engleski

Affect of molds in mixed cultures on production of aflatoxins on maize hybrid

The relationship between mold biomass and the biosynthesis of aflatoxins B1 and G1 on solid substrates (whole and crushed maize grains) at temperatures from 15-35 oC and a water content in the substrate of 20-40% has been investigated.Experiments have been carried out with the aflatoxigenous fungus Aspergillus flavus ATCC 26949 in pure and mixed cultures with latter being with the fungus Trichothecium roseum ZMPBF 1226. T. roseum does not produce aflatoxins and chromatographically similar compounds. The amount of biomass was estimated by measuring the chitin content ; the aflatoxin concentration by means of U light and ELISA method.It was established that the biosynthesis of the aflatoxins and their ratio primarily depend on the temperature of cultivation.The biomass of the mixed culture of A. flavus and T. roseum after 28 days of cultivation reduced the amount of aflatoxin B1 by 20-45%, and the amount of aflatoxin G1 by 35-50% in respect to highest values obtained in the process of biosynthesis.The decrease of both toxins is more pronounced in the substrate at the higher water contents and at the higher temperature of cultivation.

Mixed mold cultures; aflatoxins; maize hybrid; ELISA method

nije evidentirano