engleski

Expression profiles of TLR3, 7, 8 in PBMC in RSV-infected infants, healthy infants and adults

Innate immunity is important part of immune system with ability to recognize and act (neutralize) upon pathogen challenge. The engagement of Toll-like Receptors (TLR) 3, 7 and 8 within innate immunity have key role in control of viral infection and development of specific anti-viral immune reaction against RNA viruses since all three TLR recognize RNK molecules. Infants are particularly sensitive to different viral infections ; RSV represents common cause of respiratory infections with frequent re-infections related to inadequate anti-viral immunity pots-infection. Possible explanation is due less inadequate TLR3, 7, 8 engagement in innate immunity responses with lower cytokines synthesis and dendritic cell maturation (De Wit et al., J Autoimmunology 2003 ; 21:277). Using flow cytometry and specific monoclonal antibodies developed against human TLR3, 7 and 8, we determined expression profile and intensity for each receptor on isolated leukocyte subpopulations isolated from cord blood or peripheral blood of healthy infants and adults. The differences in innate immunity activation status between adults and infants could be explained by disparity in TLR expression, as one of the age dependent factors. Comparative analysis of TLR on leukocytes from peripheral blood of healthy and RSV infected infants with acute lower respiratory tract infection (bronchiolitis), might give an insight about acute anti-viral immune response. Therefore, the percentages and expression intensity for individual TLRs on monocytes, NK cells, NKT cells, T and B lymphocytes will be discussed.

respiratory syncytial virus; toll-like receptors; infants; cord blood

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