engleski

The influence of BPC 157 on nitric oxide antagonist and agonist induced lesions in broiler chicks

The main purpose of this study was to determine the effects of nitric oxide (NO) agonists and antagonists and the influence of a novel organoprotective pentadecapeptide BPC 157, on the development of the broiler pulmonary hypertension syndrome (BPHS) and tissue lesions in the chicks. Methods. Acute toxicity. The effects of single dose application of saline (1ml ip)157 (10 mikrograma /kg b.w.), L-NAME (NO-antagonist/doses 50, 100 and 150 mg/kg b.w.) with their combination (L-NAME +BPC 157, L-NAME +L-arginin/L-arginin /100 mg/kg b.w.)were investigated.Pathohistological examination of the spleen, heart, liver and lungs and haemathological analysis were conducted. Chronic toxicity. The chicks were treated daily during five weeks with L-NAME (10mg/kg b.w.), L-arginine (100 mg/kg b.w.), BPC 157 (10 mikrograma/kg b.w.) and their combination (L-NAME+ BPC 157 ; L-arginine+BPC 157) intraperitoneally (ip). Seven animals from each group, including control (saline, 1ml ip), were scarified every week. Application of L-NAME caused PBHS in treated chicks. It was prevented by simultaneous application of L-arginine and BPC 157. Pathohistological examination of both acute and chronic toxicity showed forth a severe tissue damage (myocardial and hepatocyte necrosis, necrosis of lymphoid cell in spleen) caused by L_NAME while haemorrhages, congestion and oedema were predominantly induced by l-arginine. The effect of L-NAME was successfully neutralised by the aplication of L-arginine and BPC 157. The later substance did not cause any visible tissue or organ damage. Haemathological analysis shows significant haemoglobin and leukocyte number decrease in the L-NAME treated group of chicks.

BPC 157; nitric oxide antagonist and agonist; broiler chicks

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