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TLC Determination of Chemical Interactions of Vitamins A and D with Drugs

In our work, we used thin-layer chromatography to investigate possible chemical interactions between vitamins A and D, and frequently used therapeutics (estrogens and progestins, corticosteroids, HMG CoA reductase inhibitors, vitamins, and nonsteroidal antiinmflamatory drugs). We also compared our results with biochemical interactions found in literature. Concentrations of vitamins and drugs applied to the plate were adjusted to mimic the usually prescribed doses in therapy. TLC was performed on 10 x 20 cm TLC plates, precoated with silica gel 60 F254. First step was to determine the RF values for each vitamin and drug using three different mobile phases: cyclohexane – ether, 50+50 (v/v), cyclohexane – ether, 85+15 (v/v), and ethyl acetate. Solutions of vitamins A and D were applied to the plate as 8 mm bands and investigated drugs were applied as spots. After RF values were calculated, we used a combination of chosen stationary and one mobile phase in which overlapping of vitamin band and drug spot occurred during chromatogram development. Chromatography was performed in ascending mode. The strength of interaction was measured as a surface below or above distorted part of a sample band, visible under short wave UV light (l = 254 nm) or after exposing the plates to iodine vapours. The chromatograms were documented using Camag Reprostar 3 documentation System (CAMAG, Switzerland). We established the presence of chemical interactions between vitamin A and estradiol propionate, tocoferol acetate, ibuprofen and ketoprofen, while vitamin D showed interactions with hydrocortisone, medroxyprogesterone acetate, noretisterone acetate, aspirin, ketoprofen, and acetaminophen. Clear relationship between chemical and biochemical interactions could not be established.

TLC; Chemical interactions; Vitamin A; Vitamin D

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