engleski

Immunohistochemical expression of tumour antigens MAGE-A1, MAGE-A3/4 and NY-ESO-1 in penile carcinoma

Objective: To investigated immunohistochemical expression of MAGE-A and NY-ESO-1/LAGE-1, cancer testis antigens (CTA) in penile carcinoma tissue. Methods: 30 penile carcinoma samples from patients undergoing penile amputation at the Urology Clinics at the Zagreb Clinical Hospital Center and Clinical Hospital "Sestre milosrdnice" from 1997 to 2004 were investigated in this study. Three monoclonal antibodies were used for immunohistochemical staining: 77B for MAGE-A1, 57B for multi-MAGE-A and D8.38 for NY-ESO-1 expression. Results: Expression of MAGE-A1 was observed in 13% of carcinoma samples, whereas both multi-MAGE-A and NY-ESO-1 stained 97% of samples (p<0.01). Immunohistochemical staining was only detectable in the cytoplasm. A significant heterogeneity could be observed within a same tissue sample where areas with strong positivities coexisted with cancer testis antigens negative areas. CTA expression was not significantly correlated with tumour grade. Conclusion:This study documents for the first time the expression of CTA in penis cancers. Further research is warranted with potential implications regarding both diagnosis and therapy.

MAGE-A1; MAGE-A3/4 and NY-ESO-1; penile carcinoma

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