Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 267915

Aujeszky's disease virus production in disposable bioreactor


Slivac, Igor; Gaurina Srček, Višnja; Radošević, Kristina; Kmetič, Ivana; Kniewald, Zlatko
Aujeszky's disease virus production in disposable bioreactor // Journal of Biosciences, 31 (2006), 3; 363-368 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 267915 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Aujeszky's disease virus production in disposable bioreactor

Autori
Slivac, Igor ; Gaurina Srček, Višnja ; Radošević, Kristina ; Kmetič, Ivana ; Kniewald, Zlatko

Izvornik
Journal of Biosciences (0250-5991) 31 (2006), 3; 363-368

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Aujeszky’ s disease virus; baby hamster kidney cells; cell culture; disposable bioreactor; virus titre

Sažetak
Novel, disposable-bag bioreactor system that uses wave action for mixing and transferring oxygen was evaluated for BHK 21 C13 cell line growth and Aujeszky’ s disease (AD) virus production. Growth kinetics of BHK 21 C13 cells in the Wave bioreactor during 3 days to cell density of 1.82x106 cells ml-1 was determined. At the end of the 3 day culture period and cell density of 1.82x106 cells ml-1, the reactor was inoculated with 9 ml of gE- Bartha K-61 strain AD virus suspension (105.9 TCID50) with multiplicity of infection (MOI) of 0.01. After a 144 h incubation period, 400 ml of AD virus harvest was obtained with titre of 107.0 TCID50 ml-1, which corresponds to 40, 000 doses of vaccine against AD. In conclusion, the results obtained with the Wave bioreactor using BHK 21 C13 cells showed that this system can be considered as a suitable for AD virus or BHK 21 C13 cell biomass production.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
0058010
0058001

Ustanove
Prehrambeno-biotehnološki fakultet, Zagreb

Citiraj ovu publikaciju

Slivac, Igor; Gaurina Srček, Višnja; Radošević, Kristina; Kmetič, Ivana; Kniewald, Zlatko
Aujeszky's disease virus production in disposable bioreactor // Journal of Biosciences, 31 (2006), 3; 363-368 (međunarodna recenzija, članak, znanstveni)
Slivac, I., Gaurina Srček, V., Radošević, K., Kmetič, I. & Kniewald, Z. (2006) Aujeszky's disease virus production in disposable bioreactor. Journal of Biosciences, 31 (3), 363-368.
@article{article, year = {2006}, pages = {363-368}, keywords = {Aujeszky and \#8217, s disease virus, baby hamster kidney cells, cell culture, disposable bioreactor, virus titre}, journal = {Journal of Biosciences}, volume = {31}, number = {3}, issn = {0250-5991}, title = {Aujeszky's disease virus production in disposable bioreactor}, keyword = {Aujeszky and \#8217, s disease virus, baby hamster kidney cells, cell culture, disposable bioreactor, virus titre} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • CABS
  • Geo Abstracts
  • EMBASE





Contrast
Increase Font
Decrease Font
Dyslexic Font