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Pregled bibliografske jedinice broj: 267513

Prediction of bioavailability of phenolic acids by potentiometric titration method and chromatographic techniques


Mornar, Ana; Jasprica, Ivona; Medić-Šarić, Marica
Prediction of bioavailability of phenolic acids by potentiometric titration method and chromatographic techniques // 54th Annual Congress on Medicinal Plant Research : abstracts ; u: Planta Medica / Hamburger, Matthias (ur.).
Stuttgart ; New York: Georg Thieme Verlag, 2006. str. 1011-1012 (poster, međunarodna recenzija, sažetak, znanstveni)


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Naslov
Prediction of bioavailability of phenolic acids by potentiometric titration method and chromatographic techniques

Autori
Mornar, Ana ; Jasprica, Ivona ; Medić-Šarić, Marica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
54th Annual Congress on Medicinal Plant Research : abstracts ; u: Planta Medica / Hamburger, Matthias - Stuttgart ; New York : Georg Thieme Verlag, 2006, 1011-1012

Skup
Annual Congress on Medicinal Plant Research (54 ; 2006)

Mjesto i datum
Helsinki, Finska, 29.08.-02.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ADME; phenolic acids; RP-TLC; RP-HPLC; potentiometric titration

Sažetak
Early screening of physico-chemical and ADME (Absorption, Distribution, Metabolism and Elimination) properties has become the key interest in drug discovery. Molecular lipophilicity is a major physico-chemical property, which describes oral absorption, cell uptake, protein binding, blood-brain penetration and metabolism of the bioactive substances. Phenolic acids (a group of molecules present in majority of plants) exhibit protective effects against many diseases, but whether they can reach their sites of action, particularly in humans, is largely unknown. The aim of our work was to investigate several ADME parameters of 8 phenolic acids by chromatographic techniques (RP-TLC and RP-HPLC) and potentiometric titration method. Nowadays chromatography has a tendency to replace tedious ˝shake-flask˝ method for measuring lipophilicity. The TLC measurements were performed on 10x20 cm glass plates precoated with RP-18F254s. The HPLC measurements were performed using Agilent 1100 LC System, with ZORBAX SB-C18, 4.6x150 mm, 3.5 μ m particle size column. The binary solvent system, methanol– phosphate buffer (pH=2.5), was used as a mobile phase in both techniques with a varying content of organic modifier (80-5%). The potentiometric titration method was chosen to get detailed information on the partitioning characteristics of target compounds at all pH values. pKa values and lipophilic pH-profiles were determined using Sirius instrument GLpKa. Linear regression has shown good correlation between RM(TLC) and logkw(HPLC) values (r=0.96). The correlation between experimental data and ADME parameters predicted by different computer programs was considered in order to evaluate the predictive power of the theoretical approaches applied to predict the lipophilicity of phenolic acids. Moderate correlations (r<0.90) between experimental and theoretical values demand constant evaluation of calculated data validity.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija



POVEZANOST RADA


Projekti:
0006541

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb


Citiraj ovu publikaciju:

Mornar, Ana; Jasprica, Ivona; Medić-Šarić, Marica
Prediction of bioavailability of phenolic acids by potentiometric titration method and chromatographic techniques // 54th Annual Congress on Medicinal Plant Research : abstracts ; u: Planta Medica / Hamburger, Matthias (ur.).
Stuttgart ; New York: Georg Thieme Verlag, 2006. str. 1011-1012 (poster, međunarodna recenzija, sažetak, znanstveni)
Mornar, A., Jasprica, I. & Medić-Šarić, M. (2006) Prediction of bioavailability of phenolic acids by potentiometric titration method and chromatographic techniques. U: Hamburger, M. (ur.)54th Annual Congress on Medicinal Plant Research : abstracts ; u: Planta Medica.
@article{article, author = {Mornar, Ana and Jasprica, Ivona and Medi\'{c}-\v{S}ari\'{c}, Marica}, editor = {Hamburger, M.}, year = {2006}, pages = {1011-1012}, keywords = {ADME, phenolic acids, RP-TLC, RP-HPLC, potentiometric titration}, title = {Prediction of bioavailability of phenolic acids by potentiometric titration method and chromatographic techniques}, keyword = {ADME, phenolic acids, RP-TLC, RP-HPLC, potentiometric titration}, publisher = {Georg Thieme Verlag}, publisherplace = {Helsinki, Finska} }
@article{article, author = {Mornar, Ana and Jasprica, Ivona and Medi\'{c}-\v{S}ari\'{c}, Marica}, editor = {Hamburger, M.}, year = {2006}, pages = {1011-1012}, keywords = {ADME, phenolic acids, RP-TLC, RP-HPLC, potentiometric titration}, title = {Prediction of bioavailability of phenolic acids by potentiometric titration method and chromatographic techniques}, keyword = {ADME, phenolic acids, RP-TLC, RP-HPLC, potentiometric titration}, publisher = {Georg Thieme Verlag}, publisherplace = {Helsinki, Finska} }




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