From Genes to Molecular Epidemiology (CROSBI ID 521677)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Fučić, Aleksandra ; Želježic, Davor ; Kopjar, Nevenka ; KaŠuba, Vilena ; Rozgaj, Ružica ; Ramić, Snježana ; Lucas, Joe Nathan.
engleski
From Genes to Molecular Epidemiology
Fluorescence in-situ hybridization (FISH) using whole chromosome paints is a potent tool in retrospective biological dosimetry. Generally regarded as a ‘ gold standard’ , three-color FISH is used in studies for comparison of translocation frequency with chromosome aberrations detectible by conventional cytogenetics. The aim of our work was to determine the baseline translocation yield in the peripheral blood lymphocytes using whole-chromosome paint probes for chromosomes 1, 2 and 4, and to compare it with the results of the standard chromosomal aberration analysis. The study comprised 20 individuals from the general population of the Republic Croatia (10 males and 10 females). Both gender groups were of the similar age. The mean age of the male group was 47 years (range 37 - 58), and the female group 41 (range 31 – 50). For each subject more than 1000 genome equivalents were analysed. Translocation frequencies were calculated according to Lucas et al. (1992). The translocation and chromosomal aberrations per 100 cells for male and female subjects were not significantly different, 0.41 for males and 0.3 for females. In lymphocytes of all subjects, chromatid and chromosome breaks and acentric fragments detected were in concordance with historical background values for this age group. The percentage of chromatid breaks found in males was 0.14, and in females 0.10. In females 0.1 % of chromosome breaks (0.1 % in males) and 0.07 % of acentrics (0.02 % in males) was found. Detected translocation frequency is in agreement with historical values for the analysed age group. Due to the limited number of European biodosimetry centres, the launching of common databank and standardization of selection of chromosomes for genome equivalent calculations is of great significance.
FISH; Chromosomes; Translocation; Aberrations
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
156-x.
2006.
objavljeno
Podaci o matičnoj publikaciji
From Genes to Molecular Epidemiology
Prag:
Podaci o skupu
36th Annual Meeting of the European Environmental Mutagen Society
poster
02.07.2006-06.07.2006
Prag, Češka Republika