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Pregled bibliografske jedinice broj: 266748

The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself


Aleksić, Joško; Čulo, Melanie-Ivana; Poljak, Ljiljana; Matić, Tomas; Čulo, Filip
The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself // 6th International Cytokine Conference 2006 Vienna, Austria. European Cytokine Network 1, (Suppl 2006), str.44. / Schwartzmeier Joseph (ur.).
Vienna, Austria: Medimond, Bologna, 2006. str. 1-44 (poster, međunarodna recenzija, sažetak, znanstveni)


CROSBI ID: 266748 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself

Autori
Aleksić, Joško ; Čulo, Melanie-Ivana ; Poljak, Ljiljana ; Matić, Tomas ; Čulo, Filip

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th International Cytokine Conference 2006 Vienna, Austria. European Cytokine Network 1, (Suppl 2006), str.44. / Schwartzmeier Joseph - Vienna, Austria : Medimond, Bologna, 2006, 1-44

Skup
6th International Cytokine Conference 2006

Mjesto i datum
Beč, Austrija, August 27-31, 2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
interleukin 1beta and IL-1alpha; IL-1Ra; acetaminophen; liver toxicity

Sažetak
It is known that inflammatory cytokines influence the effect of hepatotoxic chemicals on liver. Previously we have shown that IL-1alpha and IL-6 have hepatoprotective effect if given to mice before administration of acetaminophen (APAP) and that this effect is partially mediated by PGE2. In these experiments we investigated the influence of IL-1beta in the same model, since we have been able to modulate its effect with specific antagonists (IL-1Ra and polyclonal anti- IL-1beta antibodies). APAP (300 mg/kg for survival and 200 mg/kg for determination of aminotransferases) or saline in controls were administered intragastrically to mice which were given phenobarbitone in drinking water for 7 days and fasted overnight IL-1beta 500 IU /mouse) or IL-1beta antibody (0.5 ml of rabbit to mouse IL-1, obtained by three consecutive injection of IL-1beta , first in complete FCA) were given i.p. 3 hours before APAP and IL-Ra (2.5 mg/mouse) half hour before APAP. The survival of mice was followed for 72 hours and concentration aminotransferases (AST and ALT) serum were determined 18-24 hours after administration of APAP. IL-1beta significantly increased the survival mice and decreased serum level of AST and ALT (p < 0.05). Just opposite effect had IL-1beta antibodies, but the effects were not significant. Similarly IL-1Ra, significantly increased serum concentration of AST and ALT (p < 0.05 or better) (the survival was not tested in this model). The finding that that IL-1Ra alone raised serum level of aminotranferases as well that potency IL-1beta antibodies alone showed the tendency to worse the manifestations of intoxication, promoted us to analyze the synthesis if IL-1 cytokines in non-treated animals intoxicated with AAP. RT-PCR analysis of the IL-1beta and IL-1alpha expression level in liver samples from APAP intoxicated mice has revealed that acetaminophen induces the expression of both cytokines already 1 hour after its intragastric administration. This expression was even higher at 6 hours following APAP administration and could be blocked by intravenous administration of aspirin at the dose which inhibits the activity of NF-kappa B transcription factor. Based on these results we hypothesized that organism intoxicated with APAP synthesizes its own IL-1 early on following APAP administration, which would represent a host defense reaction that could be helped by applying appropriate dose of exogenous cytokines.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
108-0000000-0328 - Uloga proupalnih citokina i prostaglandina u akutnom oštećenju jetre

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Ljiljana Poljak (autor)

Avatar Url Filip Čulo (autor)

Avatar Url Melanie Ivana Čulo (autor)

Citiraj ovu publikaciju

Aleksić, Joško; Čulo, Melanie-Ivana; Poljak, Ljiljana; Matić, Tomas; Čulo, Filip
The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself // 6th International Cytokine Conference 2006 Vienna, Austria. European Cytokine Network 1, (Suppl 2006), str.44. / Schwartzmeier Joseph (ur.).
Vienna, Austria: Medimond, Bologna, 2006. str. 1-44 (poster, međunarodna recenzija, sažetak, znanstveni)
Aleksić, J., Čulo, M., Poljak, L., Matić, T. & Čulo, F. (2006) The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself. U: Schwartzmeier Joseph (ur.)6th International Cytokine Conference 2006 Vienna, Austria. European Cytokine Network 1, (Suppl 2006), str.44..
@article{article, year = {2006}, pages = {1-44}, keywords = {interleukin 1beta and IL-1alpha, IL-1Ra, acetaminophen, liver toxicity}, title = {The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself}, keyword = {interleukin 1beta and IL-1alpha, IL-1Ra, acetaminophen, liver toxicity}, publisher = {Medimond, Bologna}, publisherplace = {Be\v{c}, Austrija} }
@article{article, year = {2006}, pages = {1-44}, keywords = {interleukin 1beta and IL-1alpha, IL-1Ra, acetaminophen, liver toxicity}, title = {The protective effect of interleukin 1alpha and IL-1beta on the acetaminophen induced liver toxicity relies on the early induction of their endogenous expression by acetaminophen itself}, keyword = {interleukin 1beta and IL-1alpha, IL-1Ra, acetaminophen, liver toxicity}, publisher = {Medimond, Bologna}, publisherplace = {Be\v{c}, Austrija} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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