Activity of the aidB gene in Salmonella typhimurium (CROSBI ID 469819)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Perić, Nataša ; Bačun-Družina, Višnja
engleski
Activity of the aidB gene in Salmonella typhimurium
The adaptive response of Escherichia coli is an inducible DNA repair system that protects bacterial cells against the mutagenic and cytotoxic effects caused by alkylating agents, particularly by methylating agents. The adaptive response results in the increased expression of four genes, ada, alkA, alkB and aidB. The aidB gene of E. coli is subject of dual regulation, ada-dependent induction by alkylation and ada-independent induction by anaerobiosis or by low pH of growth medium. In contrast to the response in E. coli, an adaptive response is not detectable in Salmonella typhimurium, in spite of the presence of adaST gene in this bacterium. The aim of this study was to examine the presence of the aidB gene in S. typhimurium as well as conditions of its induction. By applying the operon fusion of E. coli aidB gene, its induction by alkylating agents, by anoxia, or by reduced pH has been determined in S. typhimurium and E. coli cells. The impact of nucleotide excision repair on the induction of aidB gene in these two bacteria has been observed. It has been certified for the first time that the aidB DNA fragment from E. coli hybridises with genomic DNA from S. typhimurium. The results of this study further confirm genomic similarity of these two related bacterial species.
Salmonella typhimurium; aidB gene; nucleotide excision repair
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
120-x.
1998.
objavljeno
Podaci o matičnoj publikaciji
Abstract book
Eckl, Peter
Salzburg: European Environmental Mutagen Society
Podaci o skupu
28th Annual Meeting
poster
07.09.1998-11.09.1998
Salzburg, Austrija