Modulation of [Ca2+]i in freshly isolated mouse lymphocytes with in vivo priming (CROSBI ID 83291)
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Forčić, Dubravko ; Mažuran, Renata
engleski
Modulation of [Ca2+]i in freshly isolated mouse lymphocytes with in vivo priming
Studied at the level of the individual cell, the pattern of [Ca2+](i) mobilization of in vivo sensitized mouse lymphocytes by T-dependent antigen (KLH), challenged in vitro by Con A, PHA or anti-CD3 epsilon mAb in different periods after immunization, was as follows. In the entire DLN lymphocyte population and in tested T cell subsets from immunized mice, baseline [Ca2+](i) was significantly increased and cells were able to respond additionally to stimuli. In KLH-primed DLN lymphocytes, calcium mobilization in response to membrane receptor-dependent stimuli (anti-CD3 epsilon, PHA, and ConA) was increased. Enhancement of Ca2+ mobilization is parallel with changed immunophenotype. These findings suggested that: (a) [Ca2+](i) mobilization could correlate with lymphocyte behaviour during immunization and that mobilization clearly depended on kinetics of immune reaction; (b) the higher level of activity among sensitized lymphocytes was due to the increased number of specific B-cells (Ia(k+)) and gamma delta TCR+ cells; (c) the quantitative measurement of [Ca2+](i) could be an important biochemical parameter to study cellular-reaction to a specific antigen.
in vivo sensitized lymphocytes; [Ca2+] (i) mobilization
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