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Pregled bibliografske jedinice broj: 265289

Delayed administration of human inter-alpha inhibitor proteins reduces mortality in sepsis


Wu, Rongqian; Cui, Xiaoxuan; Lim, Yow-Pin; Bendelja, Krešo; Zhou, Mian; Simms, Hank; Wang, Ping
Delayed administration of human inter-alpha inhibitor proteins reduces mortality in sepsis // Critical Care Medicine, 32 (2004), 8; 1747-1752 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 265289 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Delayed administration of human inter-alpha inhibitor proteins reduces mortality in sepsis

Autori
Wu, Rongqian ; Cui, Xiaoxuan ; Lim, Yow-Pin ; Bendelja, Krešo ; Zhou, Mian ; Simms, Hank ; Wang, Ping

Izvornik
Critical Care Medicine (0090-3493) 32 (2004), 8; 1747-1752

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cecal ligation and puncture; inter-alpha inhibitor proteins; survival rate; clearance; severe sepsis; half-life

Sažetak
OBJECTIVE: We have recently shown that administration of human inter-alpha inhibitor proteins (IalphaIp) very early after the onset of sepsis maintains cardiovascular stability and reduced mortality. However, it remains unknown whether injection of IalphaIp at later time points of sepsis has any beneficial effects. We therefore hypothesized that IalphaIp and its active component bikunin are reduced in sepsis and that the delayed administration of IalphaIp also improves survival rate. DESIGN:: Prospective, controlled, and randomized animal study. SETTING: A research institute laboratory. SUBJECTS:: Male adult Sprague-Dawley rats. INTERVENTIONS: Rats were subjected either to polymicrobial sepsis by cecal ligation and puncture (CLP) or to sham operation followed by the administration of normal saline solution (i.e., fluid resuscitation). MEASUREMENTS AND MAIN RESULTS:: Bikunin gene expression in the liver was measured by reverse transcription polymerase chain reaction. Plasma concentrations of IalphaIp were determined by Western blot at 5 and 20 hrs after CLP. IalphaIp clearance was assessed by injecting radioactive IalphaIp at 12 hrs post-CLP, and the half-life was determined. In addition, IalphaIp (30 mg/kg of body weight) or vehicle was administered at 1, 5, or 10 hrs (single treatment) or at both 10 and 20 hrs (double treatment) post-CLP. The necrotic cecum was excised at 20 hrs post-CLP, and 10-day survival was recorded. The results indicate that bikunin gene expression decreased significantly at 20 hrs post-CLP. Moreover, IalphaIp concentrations decreased significantly at 5 and 20 hrs post-CLP, and its half-life increased from 5.6 +/- 0.3 hrs to 11.8 +/- 2.7 hrs (p <.05), suggesting down-regulation of IalphaIp in sepsis despite the decreased clearance. Administration of IalphaIp at 1 hr post-CLP improved the survival rate from 50% to 92% (p <.05), whereas there was no significant improvement when IalphaIp was administrated at 5 or 10 hrs post-CLP. However, double injection of IalphaIp at 10 and 20 hrs post-CLP (i.e., severe sepsis) increased the survival rate from 44% to 81% (p <.05). CONCLUSION: Since delayed but repeated administration of human IalphaIp improves survival after CLP, this compound appears to be a useful agent for the treatment of severe sepsis

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti



POVEZANOST RADA


Projekti:
0021001

Ustanove:
Imunološki zavod d.d.

Profili:

Avatar Url Krešo Bendelja (autor)


Citiraj ovu publikaciju

Wu, Rongqian; Cui, Xiaoxuan; Lim, Yow-Pin; Bendelja, Krešo; Zhou, Mian; Simms, Hank; Wang, Ping
Delayed administration of human inter-alpha inhibitor proteins reduces mortality in sepsis // Critical Care Medicine, 32 (2004), 8; 1747-1752 (međunarodna recenzija, članak, znanstveni)
Wu, R., Cui, X., Lim, Y., Bendelja, K., Zhou, M., Simms, H. & Wang, P. (2004) Delayed administration of human inter-alpha inhibitor proteins reduces mortality in sepsis. Critical Care Medicine, 32 (8), 1747-1752.
@article{article, year = {2004}, pages = {1747-1752}, keywords = {cecal ligation and puncture, inter-alpha inhibitor proteins, survival rate, clearance, severe sepsis, half-life}, journal = {Critical Care Medicine}, volume = {32}, number = {8}, issn = {0090-3493}, title = {Delayed administration of human inter-alpha inhibitor proteins reduces mortality in sepsis}, keyword = {cecal ligation and puncture, inter-alpha inhibitor proteins, survival rate, clearance, severe sepsis, half-life} }
@article{article, year = {2004}, pages = {1747-1752}, keywords = {cecal ligation and puncture, inter-alpha inhibitor proteins, survival rate, clearance, severe sepsis, half-life}, journal = {Critical Care Medicine}, volume = {32}, number = {8}, issn = {0090-3493}, title = {Delayed administration of human inter-alpha inhibitor proteins reduces mortality in sepsis}, keyword = {cecal ligation and puncture, inter-alpha inhibitor proteins, survival rate, clearance, severe sepsis, half-life} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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