engleski

Electrochemical characterization of metal-binding properties of metallothioneins isolated from M. galloprovincialis

Amperometric titrations of the defined metallothionein concentrations with the standard CdCl2 solution, have been performed in seawater sample at pH 1 and pH 8, in order to define the metal-binding properties of purified mussel metallothionein component. The concentration of the formed Cd-thionein complex has been assessed in indirect and direct mode. The results on the available ligand concentration CL for complexing Cd2+ ions and the apparent stability constant K,, determined by two procedures are compared and the differences discussed. Complexing characteristics of the metallothionein can be reliably determined only if the evaluation is based on the specific signal of Cd-thionein complex.

metallothioneins; complexation of Cd2+; electrochemical method

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