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Detection of Radioprotective Effects of Propolis and Flavonoids with Cometg Assay

The use of ionizing radiation for medical diagnosis and therapy is widespread throughout the world. Recently, there has been considerable interest in finding nontoxic, commonly used substances which can protect cells from deleterious radiation effects. Formation of toxic free radicals, result in DNA damaging and mutations, changes in immunological system and theratogenic effects. Bee product, propolis and their compounds flavonoids, are potentially nontoxic radiprotectors because of their antioxidant propertis. The radioprotective effects of water-soluble derivate of propolis (WSDP), hydroalcoholic solution of propolis (HASP), quercetin, caffeic acid, chrysin and naringin in the whole-body irradiated Swiss albino and CBA mice were investigated. Animals were treated preventively and therapeuticly by intraperitoneal injection of 100 mg/kg. Irradiation was performed using a 60Co γ -ray source, and absorbed dose was 9 Gy. DNA damages in lymphocytes were detected using Comet assay in alkaline conditions. When tested, compounds were given preventively daily for three days and mice irradiated with dose of 9 Gy. Significantly DNA protection have been observed, while protection effects in therapeutic treatment were not observed. On the other hand, in nonirradiated animals, treated with tested compounds, DNA damages were not observed. Our study demonstrates that propolis and flavonoids protects DNA against the radiation induced damage.

propolis; flavonoids; radiation; radioprotection

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