Preferential integration of HPV 16 DNA in gene regions (CROSBI ID 520564)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Matovina, Mihaela ; Sabol, Ivan ; Grce, Magdalena
engleski
Preferential integration of HPV 16 DNA in gene regions
The integration of HPV DNA in the host cell genome is assumed to be the key event in the development of cervical cancer. The aim of this study was to determine the frequency of integration and the sites of viral integration in the host cell genome in HPV16 positive precancerous lesions (LSIL and HSIL). We used DIPS (Detection of Integrated Papillomavirus Sequences) PCR based method (Luft et al., Int J Cancer 2001 ; 92:9-17) with our modifications. Thirty of 176 samples were not suitable for the analysis by DIPS-PCR. In 92.5% (135/146) samples, episomal form of HPV16 DNA was determined, while integrated alone (8 cases) or mixed (3 cases) forms of HPV16 DNA were found in 7.5% (11/46) samples. In 23 LSIL samples we did not find integrated form of HPV16 DNA, while in the HSIL samples, we found integrated form in 12.3% (8 integrated and 2 mixed out of 81) samples. Also, in one sample of 42 (2.4%) with unknown cytological diagnosis mixed form of HPV16 DNA was found. The site of integration in all 11 cases was random. However, in 63.6% (7/11) cases HPV16 DNA was integrated within the common fragile sites (CFS) in the genome and in 54.5% (6/11) cases HPV16 DNA was integrated within the cellular genes, namely VMP1, CHERP, CEACAM5, PVRL1, ARID5B and AHR. Our findings confirm that HPV16 DNA integration into the host cell genome is a reliable marker of cervical neoplastic progression and that CFS, as well as gene regions, are preferred sites of HPV16 integration.
HPV
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o prilogu
262-x.
2006.
objavljeno
Podaci o matičnoj publikaciji
23rd International Papillomavirus Conference & Clinical Workshop - Book of abstracts
Hamsikova, Eva ; Haugen, Thomas H ; Palefsky, Joel ; Smith, Elaine M ; Tachezy, Ruth ; Turek, Lubomir P ; Vonka, Vladimir
Prag: The Institute of Hematology and Blood Transfusion
Podaci o skupu
23rd International Papillomavirus Conference & Clinical Workshop
poster
01.09.2006-07.09.2006
Prag, Češka Republika