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Pregled bibliografske jedinice broj: 262424

Preferential integration of HPV 16 DNA in gene regions


Matovina, Mihaela; Sabol, Ivan; Grce, Magdalena
Preferential integration of HPV 16 DNA in gene regions // 23rd International Papillomavirus Conference & Clinical Workshop - Book of abstracts / Hamsikova, Eva ; Haugen, Thomas H ; Palefsky, Joel ; Smith, Elaine M ; Tachezy, Ruth ; Turek, Lubomir P ; Vonka, Vladimir (ur.).
Prag: The Institute of Hematology and Blood Transfusion, 2006. (poster, nije recenziran, sažetak, znanstveni)


CROSBI ID: 262424 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Preferential integration of HPV 16 DNA in gene regions

Autori
Matovina, Mihaela ; Sabol, Ivan ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
23rd International Papillomavirus Conference & Clinical Workshop - Book of abstracts / Hamsikova, Eva ; Haugen, Thomas H ; Palefsky, Joel ; Smith, Elaine M ; Tachezy, Ruth ; Turek, Lubomir P ; Vonka, Vladimir - Prag : The Institute of Hematology and Blood Transfusion, 2006

Skup
23rd International Papillomavirus Conference & Clinical Workshop

Mjesto i datum
Prag, Češka Republika, 1 - 7.09.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
HPV

Sažetak
The integration of HPV DNA in the host cell genome is assumed to be the key event in the development of cervical cancer. The aim of this study was to determine the frequency of integration and the sites of viral integration in the host cell genome in HPV16 positive precancerous lesions (LSIL and HSIL). We used DIPS (Detection of Integrated Papillomavirus Sequences) PCR based method (Luft et al., Int J Cancer 2001 ; 92:9-17) with our modifications. Thirty of 176 samples were not suitable for the analysis by DIPS-PCR. In 92.5% (135/146) samples, episomal form of HPV16 DNA was determined, while integrated alone (8 cases) or mixed (3 cases) forms of HPV16 DNA were found in 7.5% (11/46) samples. In 23 LSIL samples we did not find integrated form of HPV16 DNA, while in the HSIL samples, we found integrated form in 12.3% (8 integrated and 2 mixed out of 81) samples. Also, in one sample of 42 (2.4%) with unknown cytological diagnosis mixed form of HPV16 DNA was found. The site of integration in all 11 cases was random. However, in 63.6% (7/11) cases HPV16 DNA was integrated within the common fragile sites (CFS) in the genome and in 54.5% (6/11) cases HPV16 DNA was integrated within the cellular genes, namely VMP1, CHERP, CEACAM5, PVRL1, ARID5B and AHR. Our findings confirm that HPV16 DNA integration into the host cell genome is a reliable marker of cervical neoplastic progression and that CFS, as well as gene regions, are preferred sites of HPV16 integration.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
0098104

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Magdalena Grce (autor)

Avatar Url Mihaela Matovina (autor)

Avatar Url Ivan Sabol (autor)


Citiraj ovu publikaciju:

Matovina, Mihaela; Sabol, Ivan; Grce, Magdalena
Preferential integration of HPV 16 DNA in gene regions // 23rd International Papillomavirus Conference & Clinical Workshop - Book of abstracts / Hamsikova, Eva ; Haugen, Thomas H ; Palefsky, Joel ; Smith, Elaine M ; Tachezy, Ruth ; Turek, Lubomir P ; Vonka, Vladimir (ur.).
Prag: The Institute of Hematology and Blood Transfusion, 2006. (poster, nije recenziran, sažetak, znanstveni)
Matovina, M., Sabol, I. & Grce, M. (2006) Preferential integration of HPV 16 DNA in gene regions. U: Hamsikova, E., Haugen, T., Palefsky, J., Smith, E., Tachezy, R., Turek, L. & Vonka, V. (ur.)23rd International Papillomavirus Conference & Clinical Workshop - Book of abstracts.
@article{article, author = {Matovina, Mihaela and Sabol, Ivan and Grce, Magdalena}, year = {2006}, pages = {262}, keywords = {HPV}, title = {Preferential integration of HPV 16 DNA in gene regions}, keyword = {HPV}, publisher = {The Institute of Hematology and Blood Transfusion}, publisherplace = {Prag, \v{C}e\v{s}ka Republika} }
@article{article, author = {Matovina, Mihaela and Sabol, Ivan and Grce, Magdalena}, year = {2006}, pages = {262}, keywords = {HPV}, title = {Preferential integration of HPV 16 DNA in gene regions}, keyword = {HPV}, publisher = {The Institute of Hematology and Blood Transfusion}, publisherplace = {Prag, \v{C}e\v{s}ka Republika} }




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